catalog number :
MBS260691
products type :
ELISA Kit
products full name :
Porcine Macrophage Inflammatory Protein 2 (MIP-2) ELISA Kit
products short name :
[Macrophage Inflammatory Protein 2 (MIP-2)]
other names :
[macrophage inflammatory protein 2 alpha, partial; C-X-C motif chemokine 2; C-X-C motif chemokine 2; gro-beta; MGSA beta; MIP2-alpha; GRO2 oncogene; growth-regulated protein beta; macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; chemokine (C-X-C motif) ligand 2; Growth-regulated protein beta; Gro-beta; Macrophage inflammatory protein 2-alpha; MIP2-alpha]
products gene name :
[MIP-2]
other gene names :
[CXCL2; CXCL2; GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a; GRO2; GROB; MIP2A; SCYB2; Gro-beta; MIP2-alpha; HSF]
uniprot entry name :
CXCL2_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Porcine Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Mdc Concentration. Assay Type: Sandwich. Detection Range: 1000 pg/ml-15.6 pg/ml. Sensitivity: 5 pg/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine MDC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
AAC03540.1
ncbi mol weight :
11,389 Da
ncbi pathways :
Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020)
ncbi summary :
This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CXC subfamily, is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation. [provided by RefSeq, Sep 2014]
uniprot summary :
CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family. Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q21. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; chemokine activity; CXCR chemokine receptor binding. Biological Process: G-protein coupled receptor protein signaling pathway; response to molecule of bacterial origin; immune response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; chemotaxis; inflammatory response; regulation of cell proliferation
size4 :
10x96-Strip-Wells