Human leukocyte antigen C (HLA-C) ELISA Kit | MyBioSource MBS260673 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human leukocyte antigen C (HLA-C) ELISA Kit

catalog :

MBS260673

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS260673

products type :

ELISA Kit

products full name :

Human leukocyte antigen C (HLA-C) ELISA Kit

products short name :

leukocyte antigen C (HLA-C)

other names :

leukocyte surface antigen CD47 isoform 2; Leukocyte surface antigen CD47; leukocyte surface antigen CD47; CD47 glycoprotein; Rh-related antigen; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; antigenic surface determinant protein OA3; antigen identified by monoclonal antibody 1D8; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 molecule; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD_antigen: CD47

products gene name :

HLA-C

other gene names :

CD47; CD47; IAP; OA3; MER6; MER6; IAP

uniprot entry name :

CD47_HUMAN

reactivity :

Human

sequence length :

305

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant HLA-C concentration. Assay Type: Sandwich. Detection Range: 200 U/ml-3.12 U/ml. Sensitivity: 0.6 U/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human HLA-C monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

38683836

ncbi acc num :

NP_942088.1

ncbi gb acc num :

NM_198793.2

ncbi mol weight :

33,845 Da

ncbi pathways :

Cell Surface Interactions At The Vascular Wall Pathway (106062); Cell-Cell Communication Pathway (477132); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Hemostasis Pathway (106028); Integrin Cell Surface Interactions Pathway (106110); Integrins In Angiogenesis Pathway (137999); Signal Regulatory Protein (SIRP) Family Interactions Pathway (187209); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

uniprot summary :

CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 3q13.1-q13.2. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: protein binding. Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; opsonization; positive regulation of T cell activation; cell adhesion; blood coagulation; leukocyte migration; positive regulation of inflammatory response

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage