ELISA/assay

Chicken Protein NLRC5 (NLRC5) ELISA Kit

MBS2606718

48-Strip-Wells

275 USD

ELISA Kit

Chicken Protein NLRC5 (NLRC5) ELISA Kit

[Protein NLRC5]

[protein NLRC5; Protein NLRC5; protein NLRC5; NLR family, CARD domain containing 5; Caterpiller protein 16.1; CLR16.1; Nucleotide-binding oligomerization domain protein 27; Nucleotide-binding oligomerization domain protein 4]

[NLRC5]

[NLRC5; NLRC5; NOD4; NOD27; CLR16.1; NOD27; NOD4; CLR16.1]

NLRC5_HUMAN

Chicken

1866

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Samples: Serum, plasma or cell culture supernatant. Assay Type: Quantitative Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: Up to 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Chicken NLRC5 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

350529351

NP_115582.4

NM_032206.4

170,417 Da

Immune System Pathway (1269170); Innate Immune System Pathway (1269203); Negative Regulators Of RIG-I/MDA5 Signaling Pathway (1269264); RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways (1269259)

This gene encodes a member of the caspase recruitment domain-containing NLR family. This gene plays a role in cytokine response and antiviral immunity through its inhibition of NF-kappa-B activation and negative regulation of type I interferon signaling pathways. [provided by RefSeq, Oct 2011]

NLRC5: Probable regulator of the NF-kappa-B and type I interferon signaling pathways. May also regulate the type II interferon signaling pathway. Plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms. Belongs to the NLRP family. 6 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 16q13. Cellular Component: cytoplasm; cytosol; nucleus. Molecular Function: ATP binding; protein binding. Biological Process: defense response to virus; inhibition of NF-kappaB transcription factor; innate immune response; negative regulation of interferon type I production; positive regulation of MHC class I biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; regulation of kinase activity

48-Strip-Wells

275 USD

96-Strip-Wells

435

5x96-Strip-Wells

1755

10x96-Strip-Wells

3150