Canine thrombospondin 1 (TSP-1) ELISA Kit | MyBioSource MBS2606653 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine thrombospondin 1 (TSP-1) ELISA Kit

catalog :

MBS2606653

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2606653

products type :

ELISA Kit

products full name :

Canine thrombospondin 1 (TSP-1) ELISA Kit

products short name :

[Thrombospondin 1]

other names :

[thrombospondin-1; Thrombospondin-1; thrombospondin-1; thrombospondin 1]

products gene name :

[TSP-1]

other gene names :

[THBS1; THBS1; TSP; THBS; TSP1; TSP-1; THBS-1; TSP; TSP1]

uniprot entry name :

TSP1_HUMAN

reactivity :

Canine

sequence length :

1170

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Canine serum, plasma or cell culture supernatant. Detection Range: 200 ng/ml-3.12 ng/ml. Sensitivity: 0.6/mL

other info2 :

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Canine TSP-1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

40317626

ncbi acc num :

NP_003237.2

ncbi gb acc num :

NM_003246.3

ncbi mol weight :

120,148 Da

ncbi pathways :

Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (1270244); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Hemostasis Pathway (1269340); Inflammatory Response Pathway (198766)

ncbi summary :

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]

uniprot summary :

THBS1: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties. Belongs to the thrombospondin family. Protein type: Inhibitor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 15q15. Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; external side of plasma membrane; extracellular matrix; extracellular region; extracellular space; fibrinogen complex; sarcoplasmic reticulum; secretory granule. Molecular Function: calcium ion binding; fibroblast growth factor binding; fibronectin binding; glycoprotein binding; heparin binding; identical protein binding; integrin binding; laminin binding; low-density lipoprotein binding; phosphatidylserine binding; protein binding; proteoglycan binding; transforming growth factor beta binding. Biological Process: activation of MAPK activity; behavioral response to pain; blood coagulation; cell adhesion; cell cycle arrest; cell migration; cellular protein metabolic process; chronic inflammatory response; engulfment of apoptotic cell; extracellular matrix organization and biogenesis; immune response; inflammatory response; negative regulation of angiogenesis; negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II; negative regulation of apoptosis; negative regulation of blood vessel endothelial cell migration; negative regulation of caspase activity; negative regulation of cell-matrix adhesion; negative regulation of dendritic cell antigen processing and presentation; negative regulation of endothelial cell proliferation; negative regulation of fibrinolysis; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of focal adhesion formation; negative regulation of interleukin-12 production; peptide cross-linking; platelet activation; platelet degranulation; positive regulation of angiogenesis; positive regulation of blood coagulation; positive regulation of blood vessel endothelial cell migration; positive regulation of cell migration; positive regulation of chemotaxis; positive regulation of macrophage activation; positive regulation of phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of transforming growth factor beta receptor signaling pathway; positive regulation of transforming growth factor-beta1 production; positive regulation of translation; positive regulation of tumor necrosis factor biosynthetic process; post-translational protein modification; protein amino acid O-linked glycosylation; regulation of cGMP metabolic process; response to calcium ion; response to drug; response to glucose stimulus; response to hypoxia; response to magnesium ion; response to progesterone stimulus; response to unfolded protein; sprouting angiogenesis

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

435

size3 :

5x96-Strip-Wells

price3 :

1755

size4 :

10x96-Strip-Wells

price4 :

3150

more info or order :

MyBioSource product webpage