ELISA/assay

Canine Pancreatic Polypeptide (PP) ELISA Kit

MBS2606185

48-Strip-Wells

275 USD

ELISA Kit

Canine Pancreatic Polypeptide (PP) ELISA Kit

[Pancreatic Polypeptide]

[pancreatic polypeptide; Pancreatic prohormone; pancreatic prohormone; pancreatic polypeptide; Pancreatic polypeptide; PP]

[PP]

[PPY; PPY; PP; PNP; PNP; PP; PH; PI]

PAHO_HUMAN

Canine

95

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Canine Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Piiinp Concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Canine PIIINP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

119572034

EAW51649.1

18,786 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); G Alpha (i) Signalling Events Pathway (1269576); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); Peptide Ligand-binding Receptors Pathway (1269546); Signal Transduction Pathway (1269379); Signaling By GPCR Pathway (1269543)

This gene encodes a member of the neuropeptide Y (NPY) family of peptides. The encoded 95 aa preproprotein is synthesized in the pancreatic islets of Langerhans and proteolytically processed to generate two peptide products. These products include the active pancreatic hormone of 36 aa and an icosapeptide of unknown function. This hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

PPY: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. Belongs to the NPY family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: cytoplasm; extracellular region; extracellular space. Molecular Function: G-protein-coupled receptor binding; hormone activity; neuropeptide hormone activity; receptor binding. Biological Process: cell-cell signaling; digestion; feeding behavior; neuropeptide signaling pathway; positive regulation of defense response to virus by host; protein secretion; regulation of appetite

48-Strip-Wells

275 USD

96-Strip-Wells

435

5x96-Strip-Wells

1755

10x96-Strip-Wells

3150