Goat Prostaglandin E2 (PGE2) ELISA Kit | MyBioSource MBS260610 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Goat Prostaglandin E2 (PGE2) ELISA Kit

catalog :

MBS260610

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS260610

products type :

ELISA Kit

products full name :

Goat Prostaglandin E2 (PGE2) ELISA Kit

products short name :

[Prostaglandin E2 (PGE2)]

other names :

[prostaglandin E2 receptor EP4 subtype; Prostaglandin E2 receptor EP4 subtype; prostaglandin E2 receptor EP4 subtype; prostanoid EP4 receptor; PGE receptor EP4 subtype; PGE receptor, EP4 subtype; PGE2 receptor EP4 subtype; prostaglandin E receptor 4 (subtype EP4); Prostanoid EP4 receptor]

products gene name :

[PGE2]

other gene names :

[PTGER4; PTGER4; EP4; EP4R; PTGER2; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype]

uniprot entry name :

PE2R4_HUMAN

reactivity :

Goat

sequence length :

488

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Goat Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Nk Concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Goat NK monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

4506259

ncbi acc num :

NP_000949.1

ncbi gb acc num :

NM_000958.2

ncbi mol weight :

53,119 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Eicosanoid Ligand-binding Receptors Pathway (106370); G Alpha (s) Signalling Events Pathway (119549); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Inflammatory Mediator Regulation Of TRP Channels Pathway (948277); Inflammatory Mediator Regulation Of TRP Channels Pathway (948307); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

ncbi summary :

The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor can activate T-cell factor signaling. It has been shown to mediate PGE2 induced expression of early growth response 1 (EGR1), regulate the level and stability of cyclooxygenase-2 mRNA, and lead to the phosphorylation of glycogen synthase kinase-3. Knockout studies in mice suggest that this receptor may be involved in the neonatal adaptation of circulatory system, osteoporosis, as well as initiation of skin immune responses. [provided by RefSeq, Jul 2008]

uniprot summary :

PTGER4: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function. Belongs to the G-protein coupled receptor 1 family. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1. Chromosomal Location of Human Ortholog: 5p13.1. Cellular Component: plasma membrane; integral to membrane. Molecular Function: protein binding; prostaglandin E receptor activity. Biological Process: T-helper cell differentiation; negative regulation of cytokine secretion; response to lipopolysaccharide; negative regulation of integrin activation; G-protein signaling, coupled to cAMP nucleotide second messenger; response to mechanical stimulus; negative regulation of inflammatory response; regulation of stress fiber formation; JNK cascade; immune response; regulation of ossification; positive regulation of inflammatory response; positive regulation of cytokine secretion

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

435

size3 :

5x96-Strip-Wells

price3 :

1755

size4 :

10x96-Strip-Wells

price4 :

3150

more info or order :

MyBioSource product webpage