Human Glucocorticoid Receptor Beta (GRbeta) ELISA Kit | MyBioSource MBS260594 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Glucocorticoid Receptor Beta (GRbeta) ELISA Kit

catalog :

MBS260594

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS260594

products type :

ELISA Kit

products full name :

Human Glucocorticoid Receptor Beta (GRbeta) ELISA Kit

products short name :

Glucocorticoid Receptor Beta (GRbeta)

products name syn :

Human Glucocorticoid Receptor Beta (GRb) ELISA Kit

other names :

glucocorticoid receptor isoform beta; Glucocorticoid receptor; glucocorticoid receptor; glucocorticoid nuclear receptor variant 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); Nuclear receptor subfamily 3 group C member 1

products gene name :

GRbeta

other gene names :

NR3C1; NR3C1; GR; GCR; GRL; GCCR; GCRST; GRL; GR

uniprot entry name :

GCR_HUMAN

reactivity :

Human

sequence length :

742

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant GRbeta concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human GRbeta monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

66528642

ncbi acc num :

NP_001018661.1

ncbi gb acc num :

NM_001020825.1

ncbi mol weight :

82,904 Da

ncbi pathways :

Adipogenesis Pathway (198832); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (477138); Circadian Clock Pathway (187173); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Glucocorticoid Receptor Regulatory Network Pathway (138014); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Receptor Transcription Pathway (105979)

ncbi summary :

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

uniprot summary :

GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described. Protein type: Transcription factor; Mitochondrial; DNA-binding; Nuclear receptor. Chromosomal Location of Human Ortholog: 5q31.3. Cellular Component: nucleoplasm; membrane; mitochondrial matrix; cytoplasm; nucleus; cytosol. Molecular Function: protein dimerization activity; glucocorticoid receptor activity; protein binding; zinc ion binding; transcription factor activity; steroid binding. Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; maternal behavior; adrenal gland development; regulation of glucocorticoid biosynthetic process; chromatin modification; signal transduction; glucocorticoid metabolic process; glucocorticoid mediated signaling; regulation of transcription, DNA-dependent; glucocorticoid receptor signaling pathway; positive regulation of neuron apoptosis; regulation of gluconeogenesis; positive regulation of transcription from RNA polymerase II promoter; gene expression. Disease: Glucocorticoid Resistance, Generalized

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage