ELISA/assay

Canine Norepinephrine (NE) ELISA Kit

MBS2605644

48-Strip-Wells

275 USD

ELISA Kit

Canine Norepinephrine (NE) ELISA Kit

[Norepinephrine]

[norepinephrine transporter; Sodium-dependent noradrenaline transporter; sodium-dependent noradrenaline transporter; solute carrier family 6 member 2; Norepinephrine transporter; NET; Solute carrier family 6 member 2]

[NE]

[SLC6A2; SLC6A2; NET; NAT1; NET1; SLC6A5; NAT1; NET1; SLC6A5; NET]

SC6A2_HUMAN

Canine

617

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Canine Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant N-Cad Concentration. Assay Type: Sandwich. Detection Range: 2000 pg/ml-31.2 pg/ml. Sensitivity: 12 pg/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Canine N-Cad monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

1143479

CAA62566.1

P23975

57,370 Da

Monoamine Transport Pathway (198851); Na+/Cl- Dependent Neurotransmitter Transporters Pathway (1269934); SLC-mediated Transmembrane Transport Pathway (1269907); Transmembrane Transport Of Small Molecules Pathway (1269903); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (1269923)

This gene encodes a member of the sodium:neurotransmitter symporter family. This member is a multi-pass membrane protein, which is responsible for reuptake of norepinephrine into presynaptic nerve terminals and is a regulator of norepinephrine homeostasis. Mutations in this gene cause orthostatic intolerance, a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Feb 2010]

SLC6A2: Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A2 are a cause of orthostatic intolerance (OI). OI is a syndrome characterized by lightheadedness, fatigue, altered mentation and syncope. It is associated with postural tachycardia. Plasma norepinephrine concentration is abnormally high. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily. Protein type: Membrane protein, integral; Transporter; Transporter, SLC family; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 16q12.2. Cellular Component: integral to plasma membrane; membrane; neuron projection; plasma membrane. Molecular Function: dopamine:sodium symporter activity; monoamine transmembrane transporter activity; norephinephrine:sodium symporter activity; protein binding. Biological Process: dopamine uptake; monoamine transport; norepinephrine transport; response to pain; synaptic transmission; transmembrane transport; transport. Disease: Orthostatic Intolerance

48-Strip-Wells

275 USD

96-Strip-Wells

435

5x96-Strip-Wells

1755

10x96-Strip-Wells

3150