ELISA/assay

Rat Peroxisome Proliferator Activated Receptor Alpha (PPAR-alpha) ELISA Kit

MBS260518

48-Strip-Wells

290 USD

ELISA Kit

Rat Peroxisome Proliferator Activated Receptor Alpha (PPAR-alpha) ELISA Kit

Peroxisome Proliferator Activated Receptor Alpha (PPAR-alpha)

Rat Peroxisome Proliferator Activated Receptor Alpha (PPAR-a) ELISA Kit

peroxisome proliferator activated receptor alpha; Peroxisome proliferator-activated receptor alpha; peroxisome proliferator-activated receptor alpha; PPAR-alpha; nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator-activated nuclear receptor alpha variant 3; peroxisome proliferator-activated receptor alpha; Nuclear receptor subfamily 1 group C member 1

PPAR-alpha

PPARA; PPARA; PPAR; NR1C1; hPPAR; PPARalpha; NR1C1; PPAR; PPAR-alpha

PPARA_HUMAN

Rat

468

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PPAR-alpha concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat PPAR-alpha monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

765240

AAB32649.1

18,942 Da

Activation Of Gene Expression By SREBP (SREBF) Pathway (685552); Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (198832); BMAL1:CLOCK/NPAS2 Activates Circadian Expression Pathway (477138); Circadian Clock Pathway (187173); Circadian Repression Of Expression By REV-ERBA Pathway (576259); Developmental Biology Pathway (477129); Energy Metabolism Pathway (198907); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977)

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]

PPAR-alpha: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. Belongs to the nuclear hormone receptor family. NR1 subfamily. Protein type: Nuclear receptor; DNA-binding. Chromosomal Location of Human Ortholog: 22q13.31. Cellular Component: nucleoplasm; nucleus. Molecular Function: protein domain specific binding; ligand-dependent nuclear receptor activity; NFAT protein binding; zinc ion binding; drug binding; phosphatase binding; protein binding; DNA binding; ubiquitin conjugating enzyme binding; sequence-specific DNA binding; protein complex binding; steroid hormone receptor activity; lipid binding; transcription factor activity. Biological Process: circadian rhythm; epidermis development; wound healing; positive regulation of transcription, DNA-dependent; heart development; behavioral response to nicotine; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; fatty acid metabolic process; negative regulation of appetite; response to insulin stimulus; positive regulation of gluconeogenesis; circadian regulation of gene expression; negative regulation of blood pressure; negative regulation of glycolysis; negative regulation of protein binding; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; lipoprotein metabolic process; regulation of circadian rhythm; positive regulation of fatty acid beta-oxidation; positive regulation of fatty acid oxidation; response to hypoxia; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; lipid metabolic process; fatty acid transport

48-Strip-Wells

290 USD

96-Strip-Wells

450