catalog number :
MBS260514
products type :
ELISA Kit
products full name :
Rat Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) ELISA Kit
products short name :
Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin)
other names :
visceral adipose-specific SERPIN; Serpin A12; OL-64; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin
products gene name :
Vaspin
other gene names :
SERPINA12; Vaspin
uniprot entry name :
SPA12_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant Vaspin concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat Vaspin monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
AAP88384.1
ncbi mol weight :
47,175 Da
uniprot summary :
SERPINA12: May modulate insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues. Belongs to the serpin family. Protein type: Secreted, signal peptide; Secreted; Inhibitor. Chromosomal Location of Human Ortholog: 14q32.13. Cellular Component: extracellular space; plasma membrane. Molecular Function: serine-type endopeptidase inhibitor activity. Biological Process: positive regulation of phosphoinositide 3-kinase cascade; negative regulation of lipid biosynthetic process; negative regulation of gluconeogenesis; positive regulation of insulin receptor signaling pathway