Mouse Proopiomelanocortin (POMC) ELISA Kit | MyBioSource MBS260489 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Proopiomelanocortin (POMC) ELISA Kit

catalog :

MBS260489

quantity :

48-Strip-Wells

price :

260 USD

product information

catalog number :

MBS260489

products type :

ELISA Kit

products full name :

Mouse Proopiomelanocortin (POMC) ELISA Kit

products short name :

[Proopiomelanocortin (POMC)]

other names :

[proopiomelanocortin, partial; Pro-opiomelanocortin; pro-opiomelanocortin; beta-LPH; beta-MSH; alpha-MSH; gamma-LPH; gamma-MSH; beta-endorphin; met-enkephalin; lipotropin beta; lipotropin gamma; melanotropin beta; melanotropin alpha; melanotropin gamma; pro-ACTH-endorphin; adrenocorticotropin; corticotropin-lipotropin; adrenocorticotropic hormone; opiomelanocortin prepropeptide; proopiomelanocortin preproprotein; beta-melanocyte-stimulating hormone; alpha-melanocyte-stimulating hormone; corticotropin-like intermediary peptide; proopiomelanocortin; Corticotropin-lipotropinCleaved into the following 11 chains:NPP; Melanotropin gammaAlternative name(s):Gamma-MSH]

products gene name :

[POMC]

other gene names :

[POMC; POMC; LPH; MSH; NPP; POC; ACTH; CLIP; POMC; ACTH; CLIP]

uniprot entry name :

COLI_HUMAN

reactivity :

Mouse

sequence length :

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Mouse serum, plasma or cell culture supernatant and organizations. Assay Type: Quantitative Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse POMC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

998476

ncbi acc num :

AAC60644.1

ncbi mol weight :

29,424 Da

ncbi pathways :

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Androgen Biosynthesis Pathway (106154); Biological Oxidations Pathway (105698); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Endogenous Sterols Pathway (105701); G Alpha (i) Signalling Events Pathway (119550); G Alpha (s) Signalling Events Pathway (119549); G-protein Activation Pathway (106494)

ncbi summary :

This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

POMC: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Belongs to the POMC family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 2p23.3. Cellular Component: peroxisomal matrix; extracellular space; cytoplasm; extracellular region; peroxisome; secretory granule. Molecular Function: type 3 melanocortin receptor binding; G-protein-coupled receptor binding; type 4 melanocortin receptor binding; hormone activity; receptor binding. Biological Process: generation of precursor metabolites and energy; cellular protein metabolic process; cell-cell signaling; neuropeptide signaling pathway; regulation of blood pressure; regulation of appetite; peptide hormone processing; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; signal transduction; glucose homeostasis; cellular pigmentation. Disease: Obesity; Proopiomelanocortin Deficiency

size1 :

48-Strip-Wells

price1 :

260 USD

size2 :

96-Strip-Wells

price2 :

405

size3 :

5x96-Strip-Wells

price3 :

1615

size4 :

10x96-Strip-Wells

price4 :

2880