ELISA/assay

Rat malonyl coenzyme A ELISA Kit

MBS2604885

48-Strip-Wells

260 USD

ELISA Kit

Rat malonyl coenzyme A ELISA Kit

[Malonyl coenzyme A]

[malonyl-CoA decarboxylase, mitochondrial; Malonyl-CoA decarboxylase, mitochondrial; malonyl-CoA decarboxylase, mitochondrial; malonyl-CoA decarboxylase]

[MLYCD; MLYCD; MCD; MCD]

DCMC_HUMAN

Rat

493

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Rat serum, plasma or cell culture supernatant and organizations. Assay Type: Quantitative Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat Mal CoA monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

110349750

NP_036345.2

NM_012213.2

50,946 Da

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); Peroxisomal Lipid Metabolism Pathway (1270031); Peroxisome Pathway (131226); Peroxisome Pathway (131126); Propanoate Metabolism Pathway (83004); Propanoate Metabolism Pathway (387)

The product of this gene catalyzes the breakdown of malonyl-CoA to acetyl-CoA and carbon dioxide. Malonyl-CoA is an intermediate in fatty acid biosynthesis, and also inhibits the transport of fatty acyl CoAs into mitochondria. Consequently, the encoded protein acts to increase the rate of fatty acid oxidation. It is found in mitochondria, peroxisomes, and the cytoplasm. Mutations in this gene result in malonyl-CoA decarboyxlase deficiency. [provided by RefSeq, Jul 2008]

MLYCD: Catalyzes the conversion of malonyl-CoA to acetyl-CoA. In the fatty acid biosynthesis MCD selectively removes malonyl-CoA and thus assures that methyl-malonyl-CoA is the only chain elongating substrate for fatty acid synthase and that fatty acids with multiple methyl side chains are produced. In peroxisomes it may be involved in degrading intraperoxisomal malonyl-CoA, which is generated by the peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids. Defects in MLYCD are the cause of malonyl-CoA decarboxylase deficiency (MLYCD deficiency). MLYCD deficiency is an autosomal recessive disease characterized by abdominal pain, chronic constipation, episodic vomiting, metabolic acidosis and malonic aciduria. Protein type: Lyase; EC 4.1.1.9; Carbohydrate Metabolism - propanoate; Other Amino Acids Metabolism - beta-alanine. Chromosomal Location of Human Ortholog: 16q24. Cellular Component: cytoplasm; mitochondrial matrix; mitochondrion; peroxisomal matrix; peroxisome. Molecular Function: malonyl-CoA decarboxylase activity; receptor binding. Biological Process: acetyl-CoA biosynthetic process; cellular lipid metabolic process; fatty acid biosynthetic process; positive regulation of fatty acid oxidation. Disease: Malonyl-coa Decarboxylase Deficiency

48-Strip-Wells

260 USD

96-Strip-Wells

405

5x96-Strip-Wells

1615

10x96-Strip-Wells

2880