Mouse Apolipoprotein A5 (Apo A5) ELISA Kit | MyBioSource MBS260430 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Apolipoprotein A5 (Apo A5) ELISA Kit

catalog :

MBS260430

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS260430

products type :

ELISA Kit

products full name :

Mouse Apolipoprotein A5 (Apo A5) ELISA Kit

products short name :

Apolipoprotein A5 (Apo A5)

other names :

apolipoprotein A-V; Apolipoprotein A-V; apolipoprotein A-V; apo-AV; apolipoprotein A5; regeneration-associated protein 3; apolipoprotein A-V; Apolipoprotein A5; Regeneration-associated protein 3

products gene name :

Apo A5

other gene names :

APOA5; APOA5; RAP3; APOAV; RAP3; UNQ411/PRO773; Apo-AV; ApoA-V

uniprot entry name :

APOA5_HUMAN

reactivity :

Mouse

sequence length :

366

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant Apo A5 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

. Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse Apo A5 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

262231737

ncbi gb acc num :

NP_001160070.1

ncbi mol weight :

41,213 Da

ncbi pathways :

Chylomicron-mediated Lipid Transport Pathway (106157); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450); PPARA Activates Gene Expression Pathway (530749); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (119544)

ncbi summary :

The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009]

uniprot summary :

APOA5: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Interacts with GPIHBP1. Up-regulated by PPARA agonists, which are used clinically to lower serum TG (such as fibrates). Liver and plasma. Belongs to the apolipoprotein A1/A4/E family. Protein type: Secreted; Lipid-binding; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q23. Cellular Component: extracellular space; chylomicron; extracellular region. Molecular Function: heparin binding; enzyme binding; low-density lipoprotein receptor binding; cholesterol transporter activity; enzyme activator activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; lipid binding. Biological Process: positive regulation of fatty acid biosynthetic process; organ regeneration; response to hormone stimulus; neurite regeneration; cholesterol efflux; lipoprotein metabolic process; positive regulation of lipid catabolic process; cellular lipid metabolic process; lipid transport; cholesterol biosynthetic process; positive regulation of receptor-mediated endocytosis; phosphatidylcholine metabolic process; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol metabolic process; phospholipid efflux; regulation of cholesterol absorption; tissue regeneration; acylglycerol homeostasis; triacylglycerol catabolic process; positive regulation of lipoprotein lipase activity. Disease: Hypertriglyceridemia, Familial; Hyperlipoproteinemia, Type V

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage