product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human 5-Nucleotidase (5-NT) ELISA Kit
catalog :
MBS260416
quantity :
48-Strip-Wells
price :
275 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS260416
products type :
ELISA Kit
products full name :
Human 5-Nucleotidase (5-NT) ELISA Kit
products short name :
5-Nucleotidase (5-NT)
other names :
5'-nucleotidase; 5'-nucleotidase; 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; 5' nucleotidase, ecto; Ecto-5'-nucleotidase; CD_antigen: CD73
products gene name :
5-NT
other gene names :
Nt5e; Nt5e; Nt5; CD73; Nt5; Nte; 5'-NT
uniprot entry name :
5NTD_RAT
reactivity :
Human
sequence length :
576
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant 5-NT concentration. Assay Type: Sandwich. Detection Range: 100 mIU/ml-1.56 mIU/ml. Sensitivity: 0.5 mIU/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human 5-NT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi gi num :
148747417
ncbi acc num :
NP_067587.2
ncbi gb acc num :
NM_021576.2
ncbi mol weight :
63,969 Da
ncbi pathways :
Metabolism Pathway (828668); Metabolism Of Nucleotides Pathway (828815); Nicotinate And Nicotinamide Metabolism Pathway (83406); Nicotinate And Nicotinamide Metabolism Pathway (400); Purine Catabolism Pathway (828819); Purine Metabolism Pathway (83337); Purine Metabolism Pathway (828816); Purine Metabolism Pathway (307); Pyrimidine Catabolism Pathway (828823); Pyrimidine Metabolism Pathway (83339)
ncbi summary :
may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]
uniprot summary :
NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5 -nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, GPI anchor; Nucleotide Metabolism - pyrimidine; EC 3.1.3.5; Membrane protein, integral; Phosphatase (non-protein); Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide. Cellular Component: cell surface; membrane; cytoplasm; integral to membrane; plasma membrane. Molecular Function: 5 -nucleotidase activity; ferrous iron binding; metal ion binding; nucleotide binding. Biological Process: dephosphorylation; adenosine biosynthetic process; negative regulation of inflammatory response; brain development; purine nucleotide biosynthetic process; positive regulation of lipid biosynthetic process; adenosine metabolic process; response to aluminum ion; AMP catabolic process
size1 :
48-Strip-Wells
price1 :
275 USD
size2 :
96-Strip-Wells
price2 :
420
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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