Rat Low Density Lipoprotein Receptor (LDLR) ELISA Kit | MyBioSource MBS260387 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Low Density Lipoprotein Receptor (LDLR) ELISA Kit

catalog :

MBS260387

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS260387

products type :

ELISA Kit

products full name :

Rat Low Density Lipoprotein Receptor (LDLR) ELISA Kit

products short name :

Low Density Lipoprotein Receptor (LDLR)

other names :

low density lipoprotein receptor; Low-density lipoprotein receptor; low-density lipoprotein receptor; LDL receptor; low-density lipoprotein receptor class A domain-containing protein 3; low density lipoprotein receptor

products gene name :

LDLR

other gene names :

LDLR; LDLR; FH; FHC; LDLCQ2; LDL receptor

uniprot entry name :

LDLR_HUMAN

reactivity :

Rat

sequence length :

860

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant LDLR concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

other info2 :

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat LDLR monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

307121

ncbi acc num :

AAA56833.1

ncbi mol weight :

82,255 Da

ncbi pathways :

Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Chylomicron-mediated Lipid Transport Pathway (106157); DNA Damage Response (only ATM Dependent) Pathway (198827); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907)

ncbi summary :

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

uniprot summary :

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell surface; Receptor, misc. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: Golgi apparatus; cell surface; membrane; lysosome; integral to plasma membrane; early endosome; late endosome; plasma membrane; endosome membrane; coated pit; receptor complex; external side of plasma membrane. Molecular Function: very-low-density lipoprotein receptor activity; low-density lipoprotein receptor activity; protein binding; low-density lipoprotein binding; calcium ion binding; clathrin heavy chain binding; glycoprotein binding. Biological Process: phototransduction, visible light; receptor-mediated endocytosis; lipoprotein catabolic process; cholesterol metabolic process; cholesterol transport; viral reproduction; cholesterol absorption; endocytosis; lipoprotein metabolic process; cholesterol homeostasis; phospholipid transport; lipid metabolic process; retinoid metabolic process. Disease: Hypercholesterolemia, Familial

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage