Canine Low Density Lipoprotein (LDL) ELISA Kit | MyBioSource MBS2603730 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Low Density Lipoprotein (LDL) ELISA Kit

catalog :

MBS2603730

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS2603730

products type :

ELISA Kit

products full name :

Canine Low Density Lipoprotein (LDL) ELISA Kit

products short name :

[Low Density Lipoprotein]

other names :

[low density lipoprotein, partial; Low-density lipoprotein receptor; low-density lipoprotein receptor; low density lipoprotein receptor]

products gene name :

[LDL]

other gene names :

[LDLR; LDLR; FH; FHC; LDLCQ2; LDL receptor]

uniprot entry name :

LDLR_HUMAN

reactivity :

Canine

sequence length :

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Canine Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Lam Concentration. Assay Type: Sandwich. Detection Range: 200 ng/ml-3.12 ng/ml. Sensitivity: 0.6 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Canine LAM monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

306411097

ncbi acc num :

ADM85894.1

ncbi mol weight :

82,255 Da

ncbi pathways :

Aldosterone Synthesis And Secretion Pathway (1272485); Aldosterone Synthesis And Secretion Pathway (1285075); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Chylomicron-mediated Lipid Transport Pathway (1270006); DNA Damage Response (only ATM Dependent) Pathway (198827); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907)

ncbi summary :

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

uniprot summary :

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc.; Cell surface. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: apical part of cell; basolateral plasma membrane; cell surface; coated pit; early endosome; endosome membrane; external side of plasma membrane; Golgi apparatus; integral to plasma membrane; late endosome; lysosome; membrane; plasma membrane; receptor complex. Molecular Function: calcium ion binding; clathrin heavy chain binding; glycoprotein binding; identical protein binding; low-density lipoprotein binding; low-density lipoprotein receptor activity; protease binding; protein binding; receptor activity; very-low-density lipoprotein receptor activity; viral receptor activity. Biological Process: cholesterol absorption; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; endocytosis; entry of virus into host cell; fat-soluble vitamin metabolic process; lipid metabolic process; lipoprotein catabolic process; lipoprotein metabolic process; phospholipid transport; phototransduction, visible light; receptor-mediated endocytosis; retinoid metabolic process; vitamin metabolic process. Disease: Hypercholesterolemia, Familial

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

435

size3 :

5x96-Strip-Wells

price3 :

1755

size4 :

10x96-Strip-Wells

price4 :

3150

more info or order :

MyBioSource product webpage