ELISA/assay

Rat Allantoin ELISA Kit

MBS2603517

48-Strip-Wells

260 USD

ELISA Kit

Rat Allantoin ELISA Kit

[Allantoin]

[allantoin; Protein MGF 110-14L]

[Mal-019]

11014_ASFM2

Rat

575

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Samples: Serum, plasma or cell culture supernatant. Assay Type: Quantitative Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: Up to 0.5 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat Allantoin monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

320591744

14,743 Da

ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates. Protein type: CMGC group; EC 2.7.11.24; ERK subfamily; Kinase, protein; MAPK family; MAPK/ERK subfamily; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor). Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: caveola; cytoskeleton; cytosol; early endosome; focal adhesion; Golgi apparatus; late endosome; microtubule cytoskeleton; mitochondrion; nuclear envelope; nucleoplasm; nucleus. Molecular Function: kinase activity; MAP kinase activity; phosphatase binding; protein binding; protein serine/threonine kinase activity. Biological Process: activation of MAPK activity; axon guidance; BMP signaling pathway; DNA damage induced protein phosphorylation; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; phosphorylation; platelet activation; positive regulation of cyclase activity; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of cytoskeleton organization and biogenesis; regulation of phosphoinositide 3-kinase cascade; regulation of stress-activated MAPK cascade; regulation of transcription factor activity; transcription initiation from RNA polymerase I promoter

48-Strip-Wells

260 USD

96-Strip-Wells

405

5x96-Strip-Wells

1615

10x96-Strip-Wells

2880