ELISA/assay

Canine C Reactive Protein (CRP) ELISA Kit

MBS2603188

48-Strip-Wells

275 USD

ELISA Kit

Canine C Reactive Protein (CRP) ELISA Kit

[C Reactive Protein]

[C-reactive protein; C-reactive protein; C-reactive protein; C-reactive protein, pentraxin-related]

[CRP]

[CRP; CRP; PTX1; PTX1]

CRP_HUMAN

Canine

224

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma or cell culture supernatant and organizations in the natural and recombinant CRP concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: ? 8%. Inter-assay Precision: ? 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Canine CRP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

55770842

NP_000558.2

NM_000567.2

10,415 Da

Classical Antibody-mediated Complement Activation Pathway (1269246); Complement Cascade Pathway (1269241); Creation Of C4 And C2 Activators Pathway (1269243); IL6-mediated Signaling Events Pathway (137932); Immune System Pathway (1269170); Initial Triggering Of Complement Pathway (1269242); Innate Immune System Pathway (1269203); Selenium Pathway (198825)

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1q23.2. Cellular Component: extracellular region; extracellular space. Molecular Function: calcium ion binding; choline binding; complement component C1q binding; low-density lipoprotein binding; low-density lipoprotein receptor binding; protein binding; virion binding. Biological Process: acute-phase response; defense response to Gram-positive bacterium; inflammatory response; negative regulation of vasodilation; opsonization; positive regulation of superoxide release

48-Strip-Wells

275 USD

96-Strip-Wells

435

5x96-Strip-Wells

1755

10x96-Strip-Wells

3150