catalog number :
MBS260316
products type :
ELISA Kit
products full name :
Porcine Placenta Growth Factor (PLGF) ELISA kit
products short name :
[Placenta Growth Factor (PLGF)]
other names :
[placenta growth factor isoform 1; Placenta growth factor; placenta growth factor; placental growth factor]
products gene name :
[PLGF]
other gene names :
[Pgf; Pgf; PIGF; Plgf; AI854365; Plgf; PlGF]
uniprot entry name :
PLGF_MOUSE
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Porcine serum, plasma or cell culture supernatant and organizations in the natural and recombinant PLGF concentration. Detection Range: 1000 pg/ml-15.6pg/ml. Sensitivity: Up to 5pg/ml. Assay Type: Sandwich
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine PLGF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_032853.1
ncbi gb acc num :
NM_008827.3
ncbi mol weight :
17,876 Da
ncbi pathways :
Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); PI3K-Akt Signaling Pathway (692242); PI3K-Akt Signaling Pathway (692979); Pathways In Cancer (83298); Signal Transduction Pathway (819739); Signaling By VEGF Pathway (819800); VEGF Binds To VEGFR Leading To Receptor Dimerization Pathway (819802); VEGF Ligand-receptor Interactions Pathway (819801)
uniprot summary :
PGF: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; membrane; extracellular region. Molecular Function: protein homodimerization activity; growth factor activity; protein heterodimerization activity. Biological Process: positive regulation of angiogenesis; ureteric bud branching; positive regulation of cell division; multicellular organismal development; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; sprouting angiogenesis; angiogenesis; cell differentiation
size4 :
10x96-Strip-Wells