product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Glutaminase kidney isoform, mitochondrial, GLS ELISA Kit
catalog :
MBS2602981
quantity :
48-Strip-Wells
price :
275 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS2602981
products type :
ELISA Kit
products full name :
Human Glutaminase kidney isoform, mitochondrial, GLS ELISA Kit
products short name :
glutaminase
other names :
glutaminase kidney isoform, mitochondrial isoform 2; Glutaminase kidney isoform, mitochondrial; glutaminase kidney isoform, mitochondrial; K-glutaminase; glutaminase C; L-glutamine amidohydrolase; glutaminase, phosphate-activated; glutaminase; K-glutaminase; L-glutamine amidohydrolase
other gene names :
GLS; GLS; GAC; GAM; KGA; GLS1; AAD20; GLS1; KIAA0838; GLS
uniprot entry name :
GLSK_HUMAN
reactivity :
Human
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant GLS concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156ng/ml. Sensitivity: 0.05ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human GLS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi gi num :
373251164
ncbi acc num :
NP_001243239.1
ncbi gb acc num :
NM_001256310.1
uniprot acc num :
O94925
ncbi mol weight :
65,460 Da
ncbi pathways :
Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (106173); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); D-Glutamine And D-glutamate Metabolism Pathway (82971); D-Glutamine And D-glutamate Metabolism Pathway (340); GABAergic Synapse Pathway (377263); GABAergic Synapse Pathway (377129); Glutamate Neurotransmitter Release Cycle Pathway (106522)
ncbi summary :
This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
uniprot summary :
glutaminase: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. Belongs to the glutaminase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Mitochondrial; EC 3.5.1.2; Hydrolase; Other Amino Acids Metabolism - D-Glutamine and D-glutamate; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline. Chromosomal Location of Human Ortholog: 2q32-q34. Cellular Component: mitochondrion; mitochondrial matrix; cytosol. Molecular Function: protein binding; glutaminase activity. Biological Process: synaptic transmission; glutamate secretion; suckling behavior; neurotransmitter secretion; glutamine catabolic process; neurological control of breathing; glutamate biosynthetic process; amino acid biosynthetic process; protein homotetramerization
size1 :
48-Strip-Wells
price1 :
275 USD
size2 :
96-Strip-Wells
price2 :
420
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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