ELISA/assay

Human lyso-platelet activating factor, lyso-PAF ELISA Kit

MBS2602960

48-Strip-Wells

275 USD

ELISA Kit

Human lyso-platelet activating factor, lyso-PAF ELISA Kit

lyso-platelet activating factor (lyso-PAF)

lyso-PAF acetyltransferase:LPC acyltransferase 1; Lysophosphatidylcholine acyltransferase 2; lysophosphatidylcholine acyltransferase 2; LPCAT-2; 1-AGPAT 11; LPAAT-alpha; LPC acyltransferase 2; acyltransferase like 1; acyltransferase-like 1; 1-AGP acyltransferase 11; lysoPC acyltransferase 2; lyso-PAF acetyltransferase; acetyl-CoA:lyso-PAF acetyltransferase; lysophosphatidic acid acyltransferase alpha; 1-acylglycerophosphocholine O-acyltransferase; 1-acylglycerol-3-phosphate O-acyltransferase 11; 1-alkylglycerophosphocholine O-acetyltransferase; acyl-CoA:lysophosphatidylcholine acyltransferase 2; lyso-platelet-activating factor (PAF) acetyltransferase; acetyl-CoA:lyso-platelet-activating factor acetyltransferase; lysophosphatidylcholine acyltransferase 2; 1-acylglycerol-3-phosphate O-acyltransferase 11 (EC:2.3.1.51); 1-AGP acyltransferase 11; 1-AGPAT 11; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase (EC:2.3.1.67); Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT; Acyltransferase-like 1; Lysophosphatidic acid acyltransferase alpha; LPAAT-alpha

LPCAT2; LPCAT2; AYTL1; LysoPAFAT; AGPAT11; AYTL1; LPC acyltransferase 2; LPCAT-2; LysoPC acyltransferase 2; 1-AGP acyltransferase 11; 1-AGPAT 11; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT; LPAAT-alpha

PCAT2_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant lyso-PAF concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Intended Uses: This product is suitable for in vitro quantitative detection of human serum, plasma or cell culture supernatant and organizations in the natural and recombinant lyso-PAF concentration. Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human lyso-PAF monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

126364244

BAF47696.1

Q7L5N7

30,895 Da

Acyl Chain Remodelling Of PC Pathway (645321); Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365); Glycerophospholipid Biosynthesis Pathway (645313); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Phospholipid Metabolism Pathway (645312)

This gene encodes a member of the lysophospholipid acyltransferase family. The encoded enzyme may function in two ways: to catalyze the biosynthesis of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) from 1-O-alkyl-sn-glycero-3-phosphocholine, and to catalyze the synthesis of glycerophospholipid precursors from arachidonyl-CoA and lysophosphatidylcholine. The encoded protein may function in membrane biogenesis and production of platelet-activating factor in inflammatory cells. The enzyme may localize to the endoplasmic reticulum and the Golgi. [provided by RefSeq, Feb 2009]

AYTL1: Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Involved in platelet- activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso- PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-alkyl-phosphatidylcholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.3.1.23; Membrane protein, integral; EC 2.3.1.67; EC 2.3.1.51; Acetyltransferase. Chromosomal Location of Human Ortholog: 16q12.2. Cellular Component: Golgi membrane; endoplasmic reticulum membrane; Golgi stack; endoplasmic reticulum; integral to membrane; lipid particle. Molecular Function: 1-alkylglycerophosphocholine O-acetyltransferase activity; 1-acylglycerophosphocholine O-acyltransferase activity; calcium ion binding; 1-acylglycerol-3-phosphate O-acyltransferase activity. Biological Process: platelet activating factor biosynthetic process; phospholipid metabolic process; glycerophospholipid biosynthetic process

48-Strip-Wells

275 USD

96-Strip-Wells

420