catalog number :
MBS2602946
products type :
ELISA Kit
products full name :
Rat 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta-3, PLCB3 ELISA Kit
products short name :
phospholipase C, beta 3 (phosphatidylinositol-specific)
other names :
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3; PLC-beta-3; phospholipase C beta 3; phospholipase C-beta-3; phosphoinositide phospholipase C-beta-3; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3; phospholipase C, beta 3 (phosphatidylinositol-specific); Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3
other gene names :
Plcb3; Plcb3; PLC-beta-3
uniprot entry name :
PLCB3_RAT
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Rat serum, plasma or cell culture supernatant and organizations in the natural and recombinant PLCB3 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat PLCB3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi mol weight :
139,450 Da
ncbi pathways :
African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); CFTR Activity In The Plasma Membrane Pathway (198438); Calcium Regulation In The Cardiac Cell Pathway (198495); Calcium Signaling Pathway (83442); Calcium Signaling Pathway (459)
ncbi summary :
phospholipase C isozyme activated by Gq class G proteins [RGD, Feb 2006]
uniprot summary :
PLCB3: a phosphatidylinositol-specific phospholipase C. Binds to calmodulin. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. Protein type: Carbohydrate Metabolism - inositol phosphate; EC 3.1.4.11; Phospholipase. Cellular Component: nucleoplasm; protein complex; nucleus; cytosol; sarcolemma. Molecular Function: calmodulin binding; signal transducer activity; protein binding; calcium ion binding; phospholipase C activity; phosphoinositide phospholipase C activity. Biological Process: G-protein coupled receptor protein signaling pathway; synaptic transmission; phospholipid catabolic process; regulation of systemic arterial blood pressure; post-Golgi vesicle-mediated transport; lipid catabolic process