product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Histone H3.3, H3F3A/H3.3A/H3F3/PP781H3F3B/H3.3B ELISA Kit
catalog :
MBS2602935
quantity :
48-Strip-Wells
price :
275 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS2602935
products type :
ELISA Kit
products full name :
Human Histone H3.3, H3F3A/H3.3A/H3F3/PP781H3F3B/H3.3B ELISA Kit
products short name :
H3 histone, family 3B (H3.3B)
other names :
histone H3.3; Histone H3.3; histone H3.3; H3 histone, family 3B
other gene names :
H3f3b; H3f3b; H3.3B; H3.3b
uniprot entry name :
H33_RAT
reactivity :
Human
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant H3.3/H3F3A concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human H3.3/H3F3A monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi gi num :
16758888
ncbi acc num :
NP_446437.1
ncbi gb acc num :
NM_053985.2
uniprot acc num :
P84245
ncbi mol weight :
15,328 Da
ncbi pathways :
Alcoholism Pathway (585585); Alcoholism Pathway (587116); Amyloids Pathway (1010489); Cell Cycle Pathway (1010258); Cell Cycle, Mitotic Pathway (1010278); Cellular Senescence Pathway (1010386); Cellular Responses To Stress Pathway (1010374); Condensation Of Prophase Chromosomes Pathway (1010311); Disease Pathway (1010484); Epigenetic Regulation Of Gene Expression Pathway (1010478)
ncbi summary :
histone that may be involved in brain development [RGD, Feb 2006]
uniprot summary :
H3 iso3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family. Protein type: DNA-binding. Cellular Component: nuclear chromosome; protein complex; nucleosome; nucleus. Molecular Function: nucleosomal DNA binding; DNA binding; protein heterodimerization activity. Biological Process: osteoblast differentiation; nucleosome assembly; muscle cell differentiation; DNA replication-independent nucleosome assembly; response to hormone stimulus; brain development; positive regulation of cell growth; nuclear organization and biogenesis
size1 :
48-Strip-Wells
price1 :
275 USD
size2 :
96-Strip-Wells
price2 :
420
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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