catalog number :
MBS2602934
products type :
ELISA Kit
products full name :
Human 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase eta-2, PLCH2 ELISA Kit
products short name :
phospholipase C, eta 2
other names :
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2; PLC-L4; RP3-395M20.1; phospholipase C-eta2; phospholipase C-eta-2; phospholipase C-like 4; phospholipase C-like protein 4; phosphoinositide phospholipase C-eta-2; phosphoinositide phospholipase C-like 4; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase eta-2; phospholipase C, eta 2; Phosphoinositide phospholipase C-eta-2; Phosphoinositide phospholipase C-like 4; PLC-L4; Phospholipase C-like protein 4; Phospholipase C-eta-2; PLC-eta2
other gene names :
PLCH2; PLCH2; PLCL4; PLCeta2; PLC-eta2; KIAA0450; PLCL4; PLC-L4; Phospholipase C-like protein 4; PLC-eta2
uniprot entry name :
PLCH2_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PLCH2 concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: 0.5 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
. Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PLCH2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_055453.2
ncbi gb acc num :
NM_014638.2
ncbi mol weight :
154,668 Da
ncbi summary :
PLCH2 is a member of the PLC-eta family of the phosphoinositide-specific phospholipase C (PLC) superfamily of enzymes that cleave PtdIns(4,5) P2 to generate second messengers inositol 1,4,5-trisphosphate and diacylglycerol (Zhou et al., 2005 [PubMed 16107206]).[supplied by OMIM, Jun 2009]
uniprot summary :
PLCL4: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This phospholipase activity is very sensitive to calcium. May be important for formation and maintenance of the neuronal network in the postnatal brain. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Phospholipase; EC 3.1.4.11. Chromosomal Location of Human Ortholog: 1p36.32. Cellular Component: cytoplasm; plasma membrane. Molecular Function: signal transducer activity; calcium ion binding; phosphoinositide phospholipase C activity. Biological Process: inositol phosphate metabolic process; phosphatidylinositol metabolic process; lipid catabolic process