Human Dynein light chain 1, cytoplasmic, DYNLL1/DLC1/DNCL1/DNCLC1/HDLC1 ELISA Kit | MyBioSource MBS2602921 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Dynein light chain 1, cytoplasmic, DYNLL1/DLC1/DNCL1/DNCLC1/HDLC1 ELISA Kit

catalog :

MBS2602921

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2602921

products type :

ELISA Kit

products full name :

Human Dynein light chain 1, cytoplasmic, DYNLL1/DLC1/DNCL1/DNCLC1/HDLC1 ELISA Kit

products short name :

dynein, light chain, LC8-type 1

other names :

dynein light chain 1, cytoplasmic; Dynein light chain 1, cytoplasmic; dynein light chain 1, cytoplasmic; 8 kDa dynein light chain; cytoplasmic dynein light polypeptide; dynein, cytoplasmic, light polypeptide 1; protein inhibitor of neuronal nitric oxide synthase; dynein, light chain, LC8-type 1; 8 kDa dynein light chain; DLC8; Dynein light chain LC8-type 1; Protein inhibitor of neuronal nitric oxide synthase; PIN

other gene names :

DYNLL1; DYNLL1; LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1; DLC1; DNCL1; DNCLC1; HDLC1; DLC8; PIN

uniprot entry name :

DYL1_HUMAN

reactivity :

Human

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant DYNLL1 concentration. Assay Type: Sandwich. Detection Range: 100 ng/ml-1.56 ng/ml. Sensitivity: Up to 0.5ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human DYNLL1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

83267866

ncbi acc num :

NP_001032583.1

ncbi gb acc num :

NM_001037494.1

uniprot acc num :

P63167

ncbi mol weight :

10,366 Da

ncbi pathways :

Activation Of BH3-only Proteins Pathway (105658); Activation Of BIM And Translocation To Mitochondria Pathway (105662); Adaptive Immune System Pathway (366160); Apoptosis Pathway (105648); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Centrosome Maturation Pathway (105807); G2/M Transition Pathway (105801); Immune System Pathway (106386); Intrinsic Pathway For Apoptosis (105656)

ncbi summary :

Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

LC8: a highly conserved cytoplasmic protein of the dynein light chain family. Cytoplasmic dyneins are large enzyme complexes. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain. Interacts with and inhibits the activity of neuronal nitric oxide synthase (nNOS). A highly conserved protein, showing 92% amino acid similarity with the nematode homolog. Binding of this protein destabilizes the nNOS dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Protein type: Motor; Motility/polarity/chemotaxis; Microtubule-binding. Chromosomal Location of Human Ortholog: 12q24.23. Cellular Component: signalosome; kinetochore; centrosome; microtubule; cytoplasmic dynein complex; mitochondrion; membrane; cytoplasm; plasma membrane; nucleus; cytosol. Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; protein homodimerization activity; enzyme binding; nitric-oxide synthase regulator activity; motor activity. Biological Process: anatomical structure morphogenesis; transcription, DNA-dependent; viral reproduction; apoptosis; organelle organization and biogenesis; female gamete generation; antigen processing and presentation of exogenous peptide antigen via MHC class II; microtubule-based process; substantia nigra development; regulation of transcription, DNA-dependent; transport; regulation of catalytic activity; negative regulation of phosphorylation; mitotic cell cycle; actin cytoskeleton organization and biogenesis; G2/M transition of mitotic cell cycle

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage