ELISA/assay

Human Microtubule-associated proteins 1A/1B light chain 3C, MAP1LC3C ELISA Kit

MBS2602917

48-Strip-Wells

275 USD

ELISA Kit

Human Microtubule-associated proteins 1A/1B light chain 3C, MAP1LC3C ELISA Kit

microtubule-associated protein 1 light chain 3 gamma

microtubule-associated proteins 1A/1B light chain 3C; Microtubule-associated proteins 1A/1B light chain 3C; microtubule-associated proteins 1A/1B light chain 3C; MAP1A/MAP1B LC3 C; LC3-like protein 2; MAP1A/MAP1B light chain 3 C; autophagy-related protein LC3 C; MAP1 light chain 3-like protein 2; MAP1 light chain 3-like protein 3; autophagy-related ubiquitin-like modifier LC3 C; microtubule-associated protein 1 light chain 3 gamma; Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; MAP1 light chain 3-like protein 3; MAP1A/MAP1B light chain 3 C; MAP1A/MAP1B LC3 C; Microtubule-associated protein 1 light chain 3 gamma

MAP1LC3C; MAP1LC3C; LC3C; ATG8J; MAP1A/MAP1B LC3 C

MLP3C_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant MAP1LC3C concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human MAP1LC3C monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

51972260

NP_001004343.1

NM_001004343.2

Q9BXW4

16,852 Da

Senescence And Autophagy Pathway (198780)

Autophagy is a highly regulated bulk degradation process that plays an important role in cellular maintenance and development. MAP1LC3C is an ortholog of the yeast autophagosome protein Atg8 (He et al., 2003 [PubMed 12740394]).[supplied by OMIM, Nov 2010]

LC3C: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Protein type: Ubiquitin-like modifier; Vesicle; Autophagy; Microtubule-binding. Chromosomal Location of Human Ortholog: 1q43. Cellular Component: microtubule; extrinsic to membrane; endomembrane system; autophagic vacuole; cytoplasmic vesicle; cytosol. Molecular Function: protein binding; microtubule binding; GABA receptor binding. Biological Process: mitochondrion degradation; cellular response to nitrogen starvation; autophagic vacuole formation

48-Strip-Wells

275 USD

96-Strip-Wells

420