catalog number :
MBS2602914
products type :
ELISA Kit
products full name :
Bovine Mitochondrial brown fat uncoupling protein 1, UCP1 ELISA Kit
products short name :
uncoupling protein 1 (mitochondrial, proton carrier)
other names :
Mitochondrial brown fat uncoupling protein 1; Mitochondrial brown fat uncoupling protein 1; mitochondrial brown fat uncoupling protein 1; UCP 1; thermogenin; mitochondrial, proton carrier; solute carrier family 25 member 7; uncoupling protein 1 (mitochondrial, proton carrier)<; Solute carrier family 25 member 7; Thermogenin
other gene names :
UCP1; UCP1; SLC25A7; UCP; UCP 1
uniprot entry name :
UCP1_BOVIN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: This product is suitable for in vitro quantitative detection of Bovine serum, plasma or cell culture supernatant and organizations in the natural and recombinant UCP1 concentration. Detection Range: 20 ng/ml-0.312 ng/ml
other info2 :
Sensitivity: the minimum detectable Bovine UCP1 up to 0.06 ng/ml. Intra-assay Precision: <= 8%. Inter-assay: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Bovine UCP1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi mol weight :
31,143 Da
ncbi pathways :
Electron Transport Chain Pathway 198549!!Huntington's Disease Pathway 84339!!Huntington's Disease Pathway 512!!Metabolism Pathway 581638!!Mitochondrial Uncoupling Proteins Pathway 581733!!PPAR Signaling Pathway 84281!!PPAR Signaling Pathway 450!!Respiratory Electron Transport, ATP Synthesis By Chemiosmotic Coupling, And Heat Production By Uncoupling Proteins. Pathway 581730!!The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway 581725!!The Fatty Acid Cycling Model Pathway 581734
uniprot summary :
Function: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.