Human Niemann-Pick C1-like protein 1, NPC1L1 ELISA Kit | MyBioSource MBS2602873 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Niemann-Pick C1-like protein 1, NPC1L1 ELISA Kit

catalog :

MBS2602873

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2602873

products type :

ELISA Kit

products full name :

Human Niemann-Pick C1-like protein 1, NPC1L1 ELISA Kit

products short name :

NPC1 (Niemann-Pick disease, type C1, gene) -like 1

other names :

Niemann-Pick C1-like protein 1 isoform 2; Niemann-Pick C1-like protein 1; Niemann-Pick C1-like protein 1; NPC1 (Niemann-Pick disease, type C1, gene)-like 1; NPC1-like 1

other gene names :

NPC1L1; NPC1L1; NPC11L1

uniprot entry name :

NPCL1_HUMAN

reactivity :

Human

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant NPC1L1 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human NPC1L1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

156231353

ncbi acc num :

NP_001095118.1

ncbi gb acc num :

NM_001101648.1

uniprot acc num :

Q9UHC9

ncbi mol weight :

148,728 Da

ncbi pathways :

Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Lipid Digestion, Mobilization, And Transport Pathway (106111); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Trafficking Of Dietary Sterols Pathway (106113); Vitamin A And Carotenoid Metabolism Pathway (198785)

ncbi summary :

The protein encoded by this gene is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

uniprot summary :

NPC1L1: Play a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorbtion. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. Interacts with RAB11A, MYO5B and RAB11FIP2. Interaction with RAB11A, MYO5B and RAB11FIP2 is required for proper transport to the plasma membrane upon cholesterol depletion. Expression is decreased in Caco-2 cells upon PPARD activation. Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle. Belongs to the patched family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 7p13. Cellular Component: cytoplasmic vesicle membrane; brush border membrane; apical plasma membrane; plasma membrane; integral to membrane. Molecular Function: protein binding; hedgehog receptor activity; myosin V binding; drug binding; Rab GTPase binding. Biological Process: response to drug; cholesterol homeostasis; cholesterol transport; cholesterol absorption; lipoprotein metabolic process; signal transduction; cholesterol biosynthetic process; sterol transport

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage