ELISA/assay

Mouse Inhibitor of nuclear factor kappa-B kinase subunit alpha, CHUK ELISA Kit

MBS2602786

96 Strip Wells

450 USD

ELISA Kit

Mouse Inhibitor of nuclear factor kappa-B kinase subunit alpha, CHUK ELISA Kit

conserved helix-loop-helix ubiquitous kinase

PREDICTED: inhibitor of nuclear factor kappa-B kinase subunit alpha isoform X1; Inhibitor of nuclear factor kappa-B kinase subunit alpha; inhibitor of nuclear factor kappa-B kinase subunit alpha; IKK-A; IKK alpha; I kappa B kinase 1; I-kappa-B kinase 1; IkappaB kinase alpha; I-kappa-B kinase alpha; nuclear factor NF-kappa-B inhibitor kinase alpha; conserved helix-loop-helix ubiquitous kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA

Chuk; Chuk; IKK1; Ikka; Chuk1; Fbx24; IKBKA; Fbxo24; NFKBIKA; AI256658; Ikka; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA

IKKA_MOUSE

Mouse

Store all reagents at 2-8 degree C.

569006285

XP_006526686.1

XM_006526623.1

Q60680

84,729 Da

AKT Phosphorylates Targets In The Cytosol Pathway 574945!!Activated TLR4 Signalling Pathway 575140!!Acute Myeloid Leukemia Pathway 83310!!Acute Myeloid Leukemia Pathway 529!!Adaptive Immune System Pathway 575096!!Adipocytokine Signaling Pathway 83290!!Adipocytokine Signaling Pathway 505!!Apoptosis Pathway 198339!!Apoptosis Pathway 83257!!Apoptosis Pathway 470

Function: Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3.1 PublicationManual assertion based on experiment in:Ref.11. Catalytic activity: ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein]. Enzyme regulation: Activated when phosphorylated and inactivated when dephosphorylated.

96 Strip Wells

450 USD