Human 1-acyl-sn-glycerol-3-phosphate acyltransferase beta, AGPAT2 ELISA Kit | MyBioSource MBS2602770 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human 1-acyl-sn-glycerol-3-phosphate acyltransferase beta, AGPAT2 ELISA Kit

catalog :

MBS2602770

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2602770

products type :

ELISA Kit

products full name :

Human 1-acyl-sn-glycerol-3-phosphate acyltransferase beta, AGPAT2 ELISA Kit

products short name :

1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)

other names :

1-acyl-sn-glycerol-3-phosphate acyltransferase beta isoform b; 1-acyl-sn-glycerol-3-phosphate acyltransferase beta; 1-acyl-sn-glycerol-3-phosphate acyltransferase beta; 1-AGPAT 2; 1-AGP acyltransferase 2; lysophosphatidic acid acyltransferase beta; lysophosphatidic acid acyltransferase-beta; 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta); 1-acylglycerol-3-phosphate O-acyltransferase 2; 1-acylglycerol-3-phosphate O-acyltransferase 2; 1-AGP acyltransferase 2; 1-AGPAT 2; Lysophosphatidic acid acyltransferase beta; LPAAT-beta

other gene names :

AGPAT2; AGPAT2; BSCL; BSCL1; LPAAB; 1-AGPAT2; LPAAT-beta; 1-AGP acyltransferase 2; 1-AGPAT 2; LPAAT-beta

uniprot entry name :

PLCB_HUMAN

reactivity :

Human

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant AGPAT2 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human AGPAT2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

69122971

ncbi acc num :

NP_001012745.1

ncbi gb acc num :

NM_001012727.1

uniprot acc num :

O15120

ncbi mol weight :

30,914 Da

ncbi pathways :

Adipogenesis Pathway (198832); CDP-diacylglycerol Biosynthesis I Pathway (142255); CDP-diacylglycerol Biosynthesis I Pathway (138606); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364)

ncbi summary :

This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in this gene have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

AGPAT2: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Defects in AGPAT2 are the cause of congenital generalized lipodystrophy type 1 (CGL1); also known as Berardinelli-Seip congenital lipodystrophy type 1 (BSCL1) or Berardinelli-Seip syndrome. CGL1 is an autosomal recessive disorder characterized by marked paucity of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Lipid Metabolism - glycerophospholipid; Membrane protein, multi-pass; Lipid Metabolism - glycerolipid; Transferase; Membrane protein, integral; Lipid Metabolism - ether lipid; EC 2.3.1.51. Chromosomal Location of Human Ortholog: 9q34.3. Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane. Molecular Function: 1-acylglycerol-3-phosphate O-acyltransferase activity. Biological Process: positive regulation of cytokine production; epidermis development; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; positive regulation of cytokine and chemokine mediated signaling pathway; CDP-diacylglycerol biosynthetic process. Disease: Lipodystrophy, Congenital Generalized, Type 1

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage