Human Interferon-induced, double-stranded RNA-activated protein kinase, EIF2AK2 ELISA Kit | MyBioSource MBS2602760 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Interferon-induced, double-stranded RNA-activated protein kinase, EIF2AK2 ELISA Kit

catalog :

MBS2602760

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2602760

products type :

ELISA Kit

products full name :

Human Interferon-induced, double-stranded RNA-activated protein kinase, EIF2AK2 ELISA Kit

products short name :

Interferon-induced, double-stranded RNA-activated protein kinase, EIF2AK2

other names :

interferon-induced, double-stranded RNA-activated protein kinase isoform a; Interferon-induced, double-stranded RNA-activated protein kinase; interferon-induced, double-stranded RNA-activated protein kinase; p68 kinase; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; tyrosine-protein kinase EIF2AK2; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; protein phosphatase 1, regulatory subunit 83; protein kinase, interferon-inducible double stranded RNA dependent; eukaryotic translation initiation factor 2-alpha kinase 2; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated; PKR; Tyrosine-protein kinase EIF2AK2 (EC:2.7.10.2); p68 kinase

products gene name :

EIF2AK2

other gene names :

EIF2AK2; EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83; PKR; PRKR; eIF-2A protein kinase 2; PKR

uniprot entry name :

E2AK2_HUMAN

reactivity :

Human

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant EIF2AK2 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human EIF2AK2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

208431827

ncbi acc num :

NP_001129123.1

ncbi gb acc num :

NM_001135651.2

uniprot acc num :

P19525

ncbi mol weight :

62,094 Da

ncbi pathways :

Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Ceramide Signaling Pathway (138023); Cytokine Signaling In Immune System Pathway (366171); Disease Pathway (530764); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865)

ncbi summary :

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

uniprot summary :

PKR: a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis. Controls the activation of several transcription factors such as NF-kappaB, p53 and Stats. Mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation. Protein type: Kinase, protein; Translation; Protein kinase, Other; EC 2.7.10.2; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; PEK family. Chromosomal Location of Human Ortholog: 2p22-p21. Cellular Component: membrane; perinuclear region of cytoplasm; cytoplasm; ribosome; nucleus; cytosol. Molecular Function: protein serine/threonine kinase activity; protein binding; double-stranded RNA binding; non-membrane spanning protein tyrosine kinase activity; protein phosphatase type 2A regulator activity; eukaryotic translation initiation factor 2alpha kinase activity; ATP binding; protein kinase activity. Biological Process: positive regulation of cytokine production; peptidyl-tyrosine phosphorylation; transcription, DNA-dependent; translation; activation of MAPKK activity; unfolded protein response; response to virus; protein amino acid autophosphorylation; viral infectious cycle; protein amino acid phosphorylation; evasion by virus of host immune response; positive regulation of stress-activated MAPK cascade; positive regulation of chemokine production; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; negative regulation of viral genome replication; modification by virus of host cellular process; negative regulation of translation; virus-host interaction; innate immune response; negative regulation of osteoblast proliferation; defense response to virus; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage