ELISA/assay

Human Autophagy protein 5, ATG5 ELISA Kit

MBS2602759

48-Strip-Wells

275 USD

ELISA Kit

Human Autophagy protein 5, ATG5 ELISA Kit

Autophagy protein 5, ATG5

autophagy protein 5 isoform a; Autophagy protein 5; autophagy protein 5; apoptosis-specific protein; ATG5 autophagy related 5 homolog; autophagy related 5; APG5-like; Apoptosis-specific protein

ATG5

ATG5; ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE; APG5L; ASP

ATG5_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant ATG5 concentration. Assay Type: Sandwich. Detection Range: 50 ng/ml-0.78 ng/ml. Sensitivity: 0.1 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ATG5 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

554790362

NP_001273035.1

NM_001286106.1

Q9H1Y0

32,447 Da

Immune System Pathway (106386); Innate Immune System Pathway (106387); Negative Regulators Of RIG-I/MDA5 Signaling Pathway (187102); RIG-I-like Receptor Signaling Pathway (117292); RIG-I-like Receptor Signaling Pathway (116128); RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways (187097); Regulation Of Autophagy Pathway (83058); Regulation Of Autophagy Pathway (468); Senescence And Autophagy Pathway (198780); Shigellosis Pathway (83104)

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Apoptosis; Autophagy; Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 6q21. Cellular Component: membrane; pre-autophagosomal structure membrane; cytoplasm; autophagic vacuole; axoneme. Molecular Function: protein binding; APG8 conjugating enzyme activity. Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; autophagy; innate immune response; negative regulation of protein ubiquitination; blood vessel remodeling; negative regulation of interferon type I production; otolith development; negative regulation of apoptosis; autophagic vacuole formation

48-Strip-Wells

275 USD

96-Strip-Wells

420