catalog number :
MBS2602758
products type :
ELISA Kit
products full name :
Mouse Vesicular acetylcholine transporter, SLC18A3 ELISA Kit
products short name :
Vesicular acetylcholine transporter, SLC18A3
other names :
Vesicular acetylcholine transporter; Vesicular acetylcholine transporter; vesicular acetylcholine transporter; solute carrier family 18 member 3; solute carrier family 18 (vesicular monoamine), member 3; Solute carrier family 18 member 3
products gene name :
SLC18A3
other gene names :
Slc18a3; Slc18a3; VAT; VAChT; Vacht; VAChT
uniprot entry name :
VACHT_MOUSE
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SLC18A3 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse SLC18A3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi mol weight :
56,615 Da
ncbi pathways :
Cholinergic Synapse Pathway (217717); Synaptic Vesicle Cycle Pathway (373902); Synaptic Vesicle Cycle Pathway (373890)
uniprot summary :
SLC18A3: Involved in acetylcholine transport into synaptic vesicles. Belongs to the major facilitator superfamily. Vesicular transporter family. Protein type: Membrane protein, integral; Transporter; Transporter, SLC family; Membrane protein, multi-pass. Cellular Component: synaptic vesicle; neuron projection; AP-1 adaptor complex; axon; cytoplasm; terminal button; AP-2 adaptor complex; cytoplasmic vesicle; nerve terminal. Molecular Function: acetylcholine transmembrane transporter activity; monoamine transmembrane transporter activity; acetylcholine binding. Biological Process: neurotransmitter transport; monoamine transport; transmembrane transport; cation transport