ELISA/assay

Human Interferon-induced protein with tetratricopeptide repeats 3, IFIT3 ELISA Kit

MBS2602757

48-Strip-Wells

275 USD

ELISA Kit

Human Interferon-induced protein with tetratricopeptide repeats 3, IFIT3 ELISA Kit

Interferon-induced protein with tetratricopeptide repeats 3, IFIT3

interferon-induced protein with tetratricopeptide repeats 3 isoform a; Interferon-induced protein with tetratricopeptide repeats 3; interferon-induced protein with tetratricopeptide repeats 3; CIG49; IFIT-3; IFIT-4; ISG-60; IFI-60K; interferon-induced 60 kDa protein; retinoic acid-induced gene G protein; interferon-induced protein with tetratricopeptide repeats 4; interferon-induced protein with tetratricopeptide repeats 3; CIG49; ISG-60; Interferon-induced 60 kDa protein; IFI-60K; Interferon-induced protein with tetratricopeptide repeats 4; IFIT-4; Retinoic acid-induced gene G protein; P60; RIG-G

IFIT3

IFIT3; IFIT3; P60; IRG2; IFI60; IFIT4; ISG60; RIG-G; CIG-49; GARG-49; CIG-49; IFI60; IFIT4; ISG60; IFIT-3; IFI-60K; IFIT-4; P60; RIG-G

IFIT3_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant IFIT3 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human IFIT3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

72534658

NP_001026853.1

NM_001031683.3

O14879

55,985 Da

Cytokine Signaling In Immune System Pathway (366171); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Alpha/beta Signaling Pathway (187104)

IFIT3: IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS- mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin- dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2. Belongs to the IFIT family. Protein type: Unknown function. Chromosomal Location of Human Ortholog: 10q24. Cellular Component: mitochondrion; cytoplasm; cytosol. Molecular Function: identical protein binding; protein binding. Biological Process: negative regulation of cell proliferation; response to virus; cytokine and chemokine mediated signaling pathway; defense response to virus; negative regulation of apoptosis

48-Strip-Wells

275 USD

96-Strip-Wells

420