catalog number :
MBS2602754
products type :
ELISA Kit
products full name :
Human Ras-related protein Rab-11B, RAB11B ELISA Kit
products short name :
Ras-related protein Rab-11B, RAB11B
other names :
ras-related protein Rab-11B; Ras-related protein Rab-11B; ras-related protein Rab-11B; GTP-binding protein YPT3; RAB11B, member of RAS oncogene family; RAB11B, member RAS oncogene family; GTP-binding protein YPT3
products gene name :
RAB11B
other gene names :
RAB11B; RAB11B; H-YPT3; YPT3
uniprot entry name :
RB11B_HUMAN
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant RAB11B concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human RAB11B monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_004209.2
ncbi gb acc num :
NM_004218.3
ncbi mol weight :
24,489 Da
ncbi pathways :
Endocytosis Pathway (102279); Endocytosis Pathway (102181); Vasopressin-regulated Water Reabsorption Pathway (143700); Vasopressin-regulated Water Reabsorption Pathway (143631)
ncbi summary :
The Ras superfamily of small GTP-binding proteins, which includes the Ras (see MIM 190020), Ral (see MIM 179550), Rho (see MIM 165390), Rap (see MIM 179520), and Rab (see MIM 179508) families, is involved in controlling a diverse set of essential cellular functions. The Rab family, including RAB11B, appears to play a critical role in regulating exocytotic and endocytotic pathways (summary by Zhu et al., 1994 [PubMed 7811277]).[supplied by OMIM, Nov 2010]
uniprot summary :
RAB11B: GTPase that modulates endosomal trafficking. Acts as a major regulator of membrane delivery during cytokinesis. Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Belongs to the small GTPase superfamily. Rab family. Protein type: G protein, monomeric, Rab; G protein; G protein, monomeric. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: synaptic vesicle; phagocytic vesicle membrane; recycling endosome; synaptic vesicle membrane; mitochondrion; recycling endosome membrane; phagocytic vesicle; cell junction. Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; myosin V binding. Biological Process: receptor recycling; regulated secretory pathway; intracellular protein transport; constitutive secretory pathway; transferrin transport; Rab protein signal transduction; melanosome transport