product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Sphingosine kinase 2, SPHK2 ELISA Kit
catalog :
MBS2602752
quantity :
48-Strip-Wells
price :
290 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS2602752
products type :
ELISA Kit
products full name :
Mouse Sphingosine kinase 2, SPHK2 ELISA Kit
products short name :
Sphingosine kinase 2, SPHK2
other names :
sphingosine kinase 2; Sphingosine kinase 2; sphingosine kinase 2; SK 2; SPK 2; Spk2, Sk2; sphingosine kinase 2
products gene name :
SPHK2
other gene names :
Sphk2; Sphk2; C76851; SK 2; SPK 2
uniprot entry name :
SPHK2_MOUSE
reactivity :
Mouse
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SPHK2 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse SPHK2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi gi num :
289191399
ncbi acc num :
NP_001166032.1
ncbi gb acc num :
NM_001172561.1
uniprot acc num :
Q9JIA7
ncbi mol weight :
65,618 Da
ncbi pathways :
Calcium Signaling Pathway (83247); Calcium Signaling Pathway (459); Fc Gamma R-mediated Phagocytosis Pathway (114297); Fc Gamma R-mediated Phagocytosis Pathway (106590); Metabolism Pathway (574739); Metabolism Of Lipids And Lipoproteins Pathway (574754); Signal Transduction Of S1P Receptor Pathway (198402); Sphingolipid De Novo Biosynthesis Pathway (574790); Sphingolipid Metabolism Pathway (83193); Sphingolipid Metabolism Pathway (574789)
ncbi summary :
This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. The gene product also enhances apoptosis in different cell types and suppresses cellular proliferation. In mast cells, the encoded protein is necessary for influx of calcium, protein kinase C activation, and cytokine production and degranulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
uniprot summary :
SPHK2: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine. Binds phosphoinositides. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Lipid Metabolism - sphingolipid; Kinase, lipid; EC 2.7.1.91. Cellular Component: membrane; lysosome; lysosomal membrane; cytosol. Molecular Function: transferase activity; D-erythro-sphingosine kinase activity; protein binding; sphinganine kinase activity; nucleotide binding; kinase activity; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity. Biological Process: blood vessel development; cell proliferation; metabolic process; positive regulation of cell proliferation; protein kinase C activation; sphinganine-1-phosphate biosynthetic process; sphingosine metabolic process; brain development; lipid phosphorylation; phosphorylation; negative regulation of apoptosis
size1 :
48-Strip-Wells
price1 :
290 USD
size2 :
96-Strip-Wells
price2 :
450
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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