catalog number :
MBS2602749
products type :
ELISA Kit
products full name :
Pig Metallothionein-1F, MT1F ELISA Kit
products short name :
Metallothionein-1F, MT1F
other names :
Metallothionein-1F; Metallothionein-1F; metallothionein-1A; metallothionein-IA; Metallothionein-IF; MT-IF
products gene name :
MT1F
other gene names :
MT1A; MT1F; MT-IA; MT-1F; MT-IF
uniprot entry name :
MT1F_PIG
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant MT1F concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human MT1F monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi mol weight :
5,955 Da
ncbi pathways :
Mineral Absorption Pathway (212235); Mineral Absorption Pathway (212220)
uniprot summary :
Function: Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.