catalog number :
MBS2602748
products type :
ELISA Kit
products full name :
Mouse Synaptotagmin-6, SYT6 ELISA Kit
products short name :
Synaptotagmin-6, SYT6
other names :
synaptotagmin-6 isoform 1; Synaptotagmin-6; synaptotagmin-6; synaptotagmin 6; synaptotagmin VI; Synaptotagmin VI; SytVI
products gene name :
SYT6
other gene names :
Syt6; Syt6; sytVI; AW048930; 3110037A08Rik; SytVI
uniprot entry name :
SYT6_MOUSE
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SYT6 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse SYT6 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_061270.2
ncbi gb acc num :
NM_018800.4
ncbi mol weight :
57,204 Da
uniprot summary :
SYT6: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. May mediate Ca(2+)- regulation of exocytosis in acrosomal reaction in sperm. Belongs to the synaptotagmin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Vesicle. Cellular Component: extrinsic to membrane; membrane; cell; cytoplasm; integral to membrane; plasma membrane; synapse; cytoplasmic vesicle; cytosol; cell junction. Molecular Function: identical protein binding; protein binding; clathrin binding; protein homodimerization activity; syntaxin binding; calcium-dependent phospholipid binding; protein heterodimerization activity; metal ion binding; calcium ion binding; calcium-dependent protein binding. Biological Process: vesicle fusion; synaptic vesicle exocytosis; calcium ion-dependent exocytosis of neurotransmitter; acrosome reaction; regulation of calcium ion-dependent exocytosis