ELISA/assay

Human Histone H2A.Z, H2AFZ ELISA Kit

MBS2602744

48-Strip-Wells

275 USD

ELISA Kit

Human Histone H2A.Z, H2AFZ ELISA Kit

Histone H2A.Z, H2AFZ

histone H2A.Z; Histone H2A.Z; histone H2A.Z; H2AZ histone; H2A histone family, member Z

H2AFZ

H2AFZ; H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1; H2AZ; H2A/z

H2AZ_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant H2AFZ concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human H2AFZ monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

4504255

NP_002097.1

NM_002106.3

P0C0S5

13,553 Da

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amyloids Pathway (366238); C-MYB Transcription Factor Network Pathway (138073); Cell Cycle Pathway (530733); Chromosome Maintenance Pathway (161048); Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway (161050); Disease Pathway (530764); Gene Expression Pathway (105937); Meiosis Pathway (477133)

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]

H2AZ: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP. Belongs to the histone H2A family. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 4q24. Cellular Component: Barr body; nuclear heterochromatin; nucleosome; nucleus. Molecular Function: protein binding; nucleosomal DNA binding; protein heterodimerization activity; chromatin DNA binding. Biological Process: positive regulation of transcription from RNA polymerase II promoter

48-Strip-Wells

275 USD

96-Strip-Wells

420