ELISA/assay

Human Tristetraprolin, ZFP36 ELISA Kit

MBS2602738

48-Strip-Wells

275 USD

ELISA Kit

Human Tristetraprolin, ZFP36 ELISA Kit

Tristetraprolin, ZFP36

tristetraprolin; Tristetraprolin; tristetraprolin; tristetraproline; zinc finger protein 36 homolog; G0/G1 switch regulatory protein 24; zinc finger protein 36, C3H type, homolog; zinc finger protein, C3H type, 36 homolog; growth factor-inducible nuclear protein NUP475; ZFP36 ring finger protein; G0/G1 switch regulatory protein 24; Growth factor-inducible nuclear protein NUP475; Protein TIS11A; TIS11; Zinc finger protein 36 homolog; Zfp-36

ZFP36

ZFP36; ZFP36; TTP; G0S24; GOS24; TIS11; NUP475; zfp-36; RNF162A; G0S24; RNF162A; TIS11A; TTP; TTP; TIS11; Zfp-36

TTP_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or cell culture supernatant and organizations in the natural and recombinant ZFP36 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human ZFP36 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

393539038

NP_003398.2

NM_003407.3

P26651

34,003 Da

Destabilization Of MRNA By Tristetraprolin (TTP) Pathway (187214); ErbB1 Downstream Signaling Pathway (138057); Gene Expression Pathway (105937); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Regulation Of MRNA Stability By Proteins That Bind AU-rich Elements Pathway (187211)

TTP: a CCCH zinc finger protein that has anti-inflammatory and arthritis suppressor activity. Regulates inflammatory responses at the post-transcriptional level. TTP deficiency in mice causes a severe inflammatory syndrome that includes autoimmunity, myeloid hyperplasia, and erosive arthritis. Binds to and destabilizes mRNA molecules with AU-rich elements (AREs). Has a high affinity for UUAUUUAUU mRNA sequences. Destabilizes tumor necrosis factor-alpha (TNF??), interleukin 2 (IL2), granulocyte-macrophage colony-stimulating factor (GM-CSF), transcription factor E47, and cyclooxygenase 2 (COX2) mRNAs. TNF?? and GM-CSF mRNAs are stabilized in TTP-deficient mice, causing a severe inflammatory syndrome. Conversely, high levels of TTP reduce inflammatory responses. TTP is a very low abundance protein that is rapidly induced by insulin, glucocorticoids, lipopolysaccharide (LPS), fetal calf serum, zinc, herpes simplex virus 1, green tea, and cinnamon. Once induced, the TTP protein is relatively stable. Has been experimentaly shown to be able to bind zinc. TTP is extensively phosphorylated. Protein type: DNA-binding; RNA-binding. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: stress granule; cytoplasm; nucleus; cytosol. Molecular Function: mRNA binding; protein binding; single-stranded RNA binding; enzyme binding; DNA binding; metal ion binding; C-C chemokine binding; AU-rich element binding; protein kinase binding. Biological Process: response to starvation; poly(A) tail shortening; negative regulation of myeloid cell differentiation; negative regulation of inflammatory response; mRNA catabolic process; miRNA-mediated gene silencing, negative regulation of translation; gene expression; regulation of tumor necrosis factor production; negative regulation of transcription from RNA polymerase II promoter; RNA destabilization; mRNA catabolic process, deadenylation-dependent decay

48-Strip-Wells

275 USD

96-Strip-Wells

420