catalog number :
MBS2602735
products type :
ELISA Kit
products full name :
Mouse Solute carrier family 13 member 5, SLC13A5 ELISA Kit
products short name :
Solute carrier family 13 member 5, SLC13A5
other names :
solute carrier family 13 member 5; Solute carrier family 13 member 5; solute carrier family 13 member 5; Na(+)/citrate cotransporter; sodium-coupled citrate transporter; sodium-dependent citrate transporter; novel solute carrier family 13 (sodium-dependent dicarboxylate transporter) (Slc13a2 or 3) member; solute carrier family 13 (sodium-dependent citrate transporter), member 5; Na(+)/citrate cotransporter; NaCT; Sodium-coupled citrate transporter; Sodium-dependent citrate transporter
products gene name :
SLC13A5
other gene names :
Slc13a5; Slc13a5; Indy; Nact; mINDY; NaC2/NaCT; Nact; NaCT
uniprot entry name :
S13A5_MOUSE
specificity :
No cross-reaction with other factors.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SLC13A5 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.
other info2 :
Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%
products description :
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse SLC13A5 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
ncbi acc num :
NP_001004148.1
ncbi gb acc num :
NM_001004148.4
ncbi mol weight :
63,823 Da
ncbi pathways :
SLC-mediated Transmembrane Transport Pathway (970707); Sodium-coupled Sulphate, Di- And Tri-carboxylate Transporters Pathway (1001348); Transmembrane Transport Of Small Molecules Pathway (970706); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (1001343)
uniprot summary :
SLC13A5: High-affinity sodium/citrate cotransporter that mediates citrate entry into cells. The transport process is electrogenic; it is the trivalent form of citrate rather than the divalent form that is recognized as a substrate. May facilitate the utilization of circulating citrate for the generation of metabolic energy and for the synthesis of fatty acids and cholesterol. Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transporter; Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family. Cellular Component: membrane; basolateral plasma membrane; plasma membrane; integral to membrane. Molecular Function: organic acid:sodium symporter activity; transporter activity; symporter activity; citrate transmembrane transporter activity; tricarboxylic acid transmembrane transporter activity; succinate transmembrane transporter activity; sodium:dicarboxylate symporter activity. Biological Process: citrate transport; transport; sodium ion transport; succinate transport; tricarboxylic acid transport; ion transport; transmembrane transport