ELISA/assay

Human Transmembrane protein 66, TMEM66 ELISA Kit

MBS2602733

48-Strip-Wells

275 USD

ELISA Kit

Human Transmembrane protein 66, TMEM66 ELISA Kit

Transmembrane protein 66, TMEM66

store-operated calcium entry-associated regulatory factor isoform 2; Store-operated calcium entry-associated regulatory factor; store-operated calcium entry-associated regulatory factor; SARAF long isoform; SARAF short isoform; transmembrane protein 66; HBV XAg-transactivated protein 3; SOCE-associated regulatory factor; HBV X-transactivated gene 3 protein; store-operated calcium entry-associated regulatory factor; HBV X-transactivated gene 3 protein; HBV XAg-transactivated protein 3; Protein FOAP-7; Transmembrane protein 66

TMEM66

SARAF; SARAF; XTP3; FOAP-7; TMEM66; HSPC035; TMEM66; XTP3; HSPC035; NPD003; PSEC0019; UNQ1967/PRO4499; SARAF; SOCE-associated regulatory factor

SARAF_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TMEM66 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human TMEM66 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

545746309

NP_001271168.1

NM_001284239.1

Q96BY9

36,975 Da

TMEM66: Negative regulator of store-operated Ca(2+) entry (SOCE) involved in protecting cells from Ca(2+) overfilling. In response to cytosolic Ca(2+) elevation after endoplasmic reticulum Ca(2+) refilling, promotes a slow inactivation of STIM (STIM1 or STIM2)- dependent SOCE activity: possibly act by facilitating the deoligomerization of STIM to efficiently turn off ORAI when the endoplasmic reticulum lumen is filled with the appropriate Ca(2+) levels, and thus preventing the overload of the cell with excessive Ca(2+) ions. Belongs to the SARAF family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 8p12. Cellular Component: integral to endoplasmic reticulum membrane. Molecular Function: protein binding. Biological Process: calcium ion transport

48-Strip-Wells

275 USD

96-Strip-Wells

420