Human FACT complex subunit SPT16, SUPT16H ELISA Kit | MyBioSource MBS2602732 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human FACT complex subunit SPT16, SUPT16H ELISA Kit

catalog :

MBS2602732

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2602732

products type :

ELISA Kit

products full name :

Human FACT complex subunit SPT16, SUPT16H ELISA Kit

products short name :

FACT complex subunit SPT16, SUPT16H

other names :

FACT complex subunit SPT16; FACT complex subunit SPT16; FACT complex subunit SPT16; hSPT16; FACT 140 kDa subunit; facilitates chromatin remodeling 140 kDa subunit; facilitates chromatin transcription complex subunit SPT16; chromatin-specific transcription elongation factor 140 kDa subunit; suppressor of Ty 16 homolog (S. cerevisiae); Chromatin-specific transcription elongation factor 140 kDa subunit; FACT 140 kDa subunit; FACTp140; Facilitates chromatin transcription complex subunit SPT16; hSPT16

products gene name :

SUPT16H

other gene names :

SUPT16H; SUPT16H; CDC68; FACTP140; SPT16/CDC68; FACT140; FACTP140; hSPT16

uniprot entry name :

SP16H_HUMAN

reactivity :

Human

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SUPT16H concentration. Assay Type: Sandwich. Detection Range: 50 ng/ml-0.78 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SUPT16H monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

6005757

ncbi acc num :

NP_009123.1

ncbi gb acc num :

NM_007192.3

uniprot acc num :

Q9Y5B9

ncbi mol weight :

119,914 Da

ncbi pathways :

Disease Pathway (530764); Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway (106004); Formation Of HIV-1 Elongation Complex In The Absence Of HIV-1 Tat Pathway (161052); Formation Of RNA Pol II Elongation Complex Pathway (105950); Gene Expression Pathway (105937); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984); HIV-1 Transcription Elongation Pathway (106001); HIV-1 Elongation Arrest And Recovery Pathway (161051); Late Phase Of HIV Life Cycle Pathway (105995)

ncbi summary :

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009]

uniprot summary :

SPT16: Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of Ser-392 of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Belongs to the peptidase M24 family. SPT16 subfamily. Component of the FACT complex, a stable heterodimer of SSRP1 and SUPT16H. Also component of a CK2-SPT16-SSRP1 complex which forms following UV irradiation, composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Interacts with NEK9. Binds to histone H2A-H2B. Interacts with GTF2E2. Protein type: Transcription initiation complex. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: nucleoplasm; chromosome; nucleus. Molecular Function: protein binding. Biological Process: transcription from RNA polymerase II promoter; viral reproduction; nucleosome disassembly; positive regulation of viral transcription; positive regulation of RNA elongation; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair; DNA replication

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage