ELISA/assay

Human Transcription factor Dp-1, TFDP1 ELISA Kit

MBS2602728

48-Strip-Wells

275 USD

ELISA Kit

Human Transcription factor Dp-1, TFDP1 ELISA Kit

Transcription factor Dp-1, TFDP1

transcription factor Dp-1; Transcription factor Dp-1; transcription factor Dp-1; DRTF1-polypeptide 1; E2F dimerization partner 1; E2F-related transcription factor; transcription factor Dp-1; DRTF1-polypeptide 1; DRTF1; E2F dimerization partner 1

TFDP1

TFDP1; TFDP1; DP1; Dp-1; DRTF1; DP1; DRTF1

TFDP1_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TFDP1 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human TFDP1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

6005900

NP_009042.1

NM_007111.4

Q14186

45,070 Da

Activation Of BH3-only Proteins Pathway (105658); Activation Of NOXA And Translocation To Mitochondria Pathway (105660); Activation Of PUMA And Translocation To Mitochondria Pathway (105661); Apoptosis Pathway (105648); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Cyclin D Associated Events In G1 Pathway (105767)

This gene encodes a member of a family of transcription factors that heterodimerize with E2F proteins to enhance their DNA-binding activity and promote transcription from E2F target genes. The encoded protein functions as part of this complex to control the transcriptional activity of numerous genes involved in cell cycle progression from G1 to S phase. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1, 15, and X.[provided by RefSeq, Jan 2009]

TFDP1: Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5 -TTTC[CG]CGC-3 , found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1/DP complex appears to mediate both cell proliferation and apoptosis. Belongs to the E2F/DP family. Protein type: Transcription, coactivator/corepressor; DNA-binding. Chromosomal Location of Human Ortholog: 13q34. Cellular Component: nucleoplasm; transcription factor complex. Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription coactivator activity; transcription factor binding; transcription factor activity. Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; epidermis development; transcription, DNA-dependent; apoptosis; anoikis; regulation of transcription from RNA polymerase II promoter; cell proliferation; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; mitotic cell cycle; G1/S transition of mitotic cell cycle

48-Strip-Wells

275 USD

96-Strip-Wells

420