Human E3 ubiquitin-protein ligase UHRF2, UHRF2 ELISA Kit | MyBioSource MBS2602716 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human E3 ubiquitin-protein ligase UHRF2, UHRF2 ELISA Kit

catalog :

MBS2602716

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2602716

products type :

ELISA Kit

products full name :

Human E3 ubiquitin-protein ligase UHRF2, UHRF2 ELISA Kit

products short name :

E3 ubiquitin-protein ligase UHRF2, UHRF2

other names :

E3 ubiquitin-protein ligase UHRF2; E3 ubiquitin-protein ligase UHRF2; E3 ubiquitin-protein ligase UHRF2; nuclear protein 97; RING finger protein 107; Np95-like ring finger protein; nuclear zinc finger protein NP97; np95/ICBP90-like RING finger protein; ubiquitin-like, containing PHD and RING finger domains, 2; ubiquitin-like PHD and RING finger domain-containing protein 2; ubiquitin-like-containing PHD and RING finger domains protein 2; ubiquitin-like with PHD and ring finger domains 2, E3 ubiquitin protein ligase; Np95/ICBP90-like RING finger protein; Np95-like RING finger protein; Nuclear protein 97; Nuclear zinc finger protein Np97; RING finger protein 107; Ubiquitin-like PHD and RING finger domain-containing protein 2; Ubiquitin-like-containing PHD and RING finger domains protein 2

products gene name :

UHRF2

other gene names :

UHRF2; UHRF2; NIRF; URF2; RNF107; NIRF; RNF107; Np95-like RING finger protein

uniprot entry name :

UHRF2_HUMAN

reactivity :

Human

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant UHRF2 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human UHRF2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

23312364

ncbi acc num :

NP_690856.1

ncbi gb acc num :

NM_152896.2

uniprot acc num :

Q96PU4

ncbi mol weight :

56,077 Da

ncbi summary :

This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis. The encoded protein contains an NIRF_N domain, a PHD finger, a set- and ring-associated (SRA) domain, and a RING finger domain and several of these domains have been shown to be essential for the regulation of cell proliferation. This protein may also have a role in intranuclear degradation of polyglutamine aggregates. Alternative splicing results in multiple transcript variants some of which are non-protein coding. [provided by RefSeq, Feb 2012]

uniprot summary :

UHRF2: E3 ubiquitin-protein ligase that is an intermolecular hub protein in the cell cycle network. Through cooperative DNA and histone binding, may contribute to a tighter epigenetic control of gene expression in differentiated cells. Ubiquitinates cyclins, CCND1 and CCNE1, in an apparently phosphorylation-independent manner and induces G1 arrest. Also ubiquitinates PCNP leading to its degradation by the proteasome. Appears to contribute to tumorigenesis. Associated with various cancers. DNA copy number loss is found in multiple kinds of malignancies originating from the brain, breast, stomach, kidney, hematopoietic tissue and lung. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation; Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-; EC 6.3.2.19; Ligase. Chromosomal Location of Human Ortholog: 9p24.1. Cellular Component: nucleoplasm; nuclear heterochromatin; nucleus. Molecular Function: protein binding; DNA binding; histone binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity. Biological Process: ubiquitin-dependent protein catabolic process; cell proliferation; protein autoubiquitination; regulation of cell cycle; protein ubiquitination; cell differentiation; cell cycle

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage