ELISA/assay

Mouse Paired box protein Pax-7, PAX7 ELISA Kit

MBS2602714

48-Strip-Wells

290 USD

ELISA Kit

Mouse Paired box protein Pax-7, PAX7 ELISA Kit

Paired box protein Pax-7, PAX7

paired box protein Pax-7; Paired box protein Pax-7; paired box protein Pax-7; paired box gene 7; paired box transcription factor PAX7; paired box 7

PAX7

Pax7; Pax7; Pax-7; Pax-7

PAX7_MOUSE

Mouse

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Mouse serum, plasma or cell culture supernatant and organizations in the natural and recombinant PAX7 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the Assay This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse PAX7 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

34328055

NP_035169.1

NM_011039.2

P47239

54,939 Da

Transcriptional Misregulation In Cancer Pathway (523020); Transcriptional Misregulation In Cancer Pathway (522987)

PAX7: Probable transcription factor. May have a role in myogenesis. Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2). It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator. Belongs to the paired homeobox family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription factor; DNA-binding; Oncoprotein. Cellular Component: transcription factor complex; nucleus. Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; skeletal muscle development; muscle development; positive regulation of histone methylation; transcription, DNA-dependent; satellite cell commitment; multicellular organismal development; embryonic skeletal development; neuron fate commitment; skeletal muscle regeneration; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; organ morphogenesis; regulation of transcription, DNA-dependent; regulation of gene expression; regulation of protein binding; cartilage development; regulation of cell fate commitment; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; dorsoventral neural tube patterning

48-Strip-Wells

290 USD

96-Strip-Wells

450