Human Shugoshin-like 1, SGOL1 ELISA Kit | MyBioSource MBS2602713 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Shugoshin-like 1, SGOL1 ELISA Kit

catalog :

MBS2602713

quantity :

48-Strip-Wells

price :

275 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2602713

products type :

ELISA Kit

products full name :

Human Shugoshin-like 1, SGOL1 ELISA Kit

products short name :

Shugoshin-like 1, SGOL1

other names :

shugoshin-like 1 isoform A1; Shugoshin-like 1; shugoshin-like 1; hSgo1; shugoshin 1AB protein; shugoshin 1CD protein; shugoshin 1EF protein; shugoshin 1GH protein; shugoshin 1KL protein; serologically defined breast cancer antigen NY-BR-85; shugoshin-like 1 (S. pombe); Serologically defined breast cancer antigen NY-BR-85

products gene name :

SGOL1

other gene names :

SGOL1; SGOL1; SGO; Sgo1; NY-BR-85; SGO1; hSgo1

uniprot entry name :

SGOL1_HUMAN

reactivity :

Human

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SGOL1 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SGOL1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

60302875

ncbi acc num :

NP_001012409.1

ncbi gb acc num :

NM_001012409.2

uniprot acc num :

Q5FBB7

ncbi mol weight :

64,190 Da

ncbi pathways :

Aurora B Signaling Pathway (138080); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); DNA Replication Pathway (105897); M Phase Pathway (105812); Mitotic M-M/G1 Phases Pathway (160943); Mitotic Prometaphase Pathway (105815); Oocyte Meiosis Pathway (126909); Oocyte Meiosis Pathway (126872); PLK1 Signaling Events Pathway (138007)

ncbi summary :

The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]

uniprot summary :

SGO1: Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. Belongs to the shugoshin family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation. Chromosomal Location of Human Ortholog: 3p24.3. Cellular Component: kinetochore; nucleoplasm; spindle pole; centrosome; cytoplasm; cytosol; condensed nuclear chromosome, pericentric region; chromosome, pericentric region. Molecular Function: protein binding; kinase binding. Biological Process: mitosis; cell division; mitotic cell cycle; attachment of spindle microtubules to kinetochore; meiotic chromosome segregation; centriole-centriole cohesion; chromosome segregation. Disease: Chronic Atrial And Intestinal Dysrhythmia

size1 :

48-Strip-Wells

price1 :

275 USD

size2 :

96-Strip-Wells

price2 :

420

more info or order :

MyBioSource product webpage