Mouse Importin subunit beta-1, KPNB1 ELISA Kit | MyBioSource MBS2602712 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Importin subunit beta-1, KPNB1 ELISA Kit

catalog :

MBS2602712

quantity :

48-Strip-Wells

price :

290 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS2602712

products type :

ELISA Kit

products full name :

Mouse Importin subunit beta-1, KPNB1 ELISA Kit

products short name :

Importin subunit beta-1, KPNB1

other names :

importin subunit beta-1; Importin subunit beta-1; importin subunit beta-1; SCG; PTAC97; importin beta; nuclear factor p97; karyopherin subunit beta-1; pore targeting complex 97 kDa subunit; karyopherin (importin) beta 1; Karyopherin subunit beta-1; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97; SCG

products gene name :

KPNB1

other gene names :

Kpnb1; Kpnb1; IPOB; Impnb; AA409963; Impnb; PTAC97

uniprot entry name :

IMB1_MOUSE

reactivity :

Mouse

specificity :

No cross-reaction with other factors.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant KPNB1 concentration. Assay Type: Sandwich. Detection Range: 50 ng/ml-0.78 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

products description :

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse KPNB1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

ncbi gi num :

88014720

ncbi acc num :

NP_032405.3

ncbi gb acc num :

NM_008379.3

uniprot acc num :

P70168

ncbi mol weight :

97,184 Da

ncbi pathways :

Activation Of DNA Fragmentation Factor Pathway (574618); Apoptosis Pathway (574586); Apoptosis Induced DNA Fragmentation Pathway (574617); Apoptotic Execution Phase Pathway (574612); PluriNetWork Pathway (198307); RNA Transport Pathway (177887); RNA Transport Pathway (175229); TGF-beta Receptor Signaling Pathway (198330)

uniprot summary :

KPNB1: a nuclear import protein nuclear protein import that functions either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re- exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus. Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. Two isoforms of the human protein have been reported. Belongs to the importin beta family. Protein type: Karyopherin; Nuclear import. Cellular Component: nucleoplasm; nuclear membrane; protein complex; membrane; cell; cytoplasm; nuclear envelope; intracellular; nucleus. Molecular Function: protein domain specific binding; protein binding; enzyme binding; Ran GTPase binding; protein transporter activity. Biological Process: intracellular protein transport; ribosomal protein import into nucleus; protein transport; protein import into nucleus; transport; nucleocytoplasmic transport

size1 :

48-Strip-Wells

price1 :

290 USD

size2 :

96-Strip-Wells

price2 :

450

more info or order :

MyBioSource product webpage