ELISA/assay

Human E3 ubiquitin-protein ligase TRAF7, TRAF7 ELISA Kit

MBS2602709

48-Strip-Wells

275 USD

ELISA Kit

Human E3 ubiquitin-protein ligase TRAF7, TRAF7 ELISA Kit

E3 ubiquitin-protein ligase TRAF7, TRAF7

E3 ubiquitin-protein ligase TRAF7; E3 ubiquitin-protein ligase TRAF7; E3 ubiquitin-protein ligase TRAF7; RING finger protein 119; ring finger and WD repeat domain 1; RING finger and WD repeat-containing protein 1; TNF receptor-associated factor 7, E3 ubiquitin protein ligase; RING finger and WD repeat-containing protein 1; RING finger protein 119; TNF receptor-associated factor 7

TRAF7

TRAF7; TRAF7; RFWD1; RNF119; RFWD1; RNF119

TRAF7_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TRAF7 concentration. Assay Type: Sandwich. Detection Range: 20 ng/ml-0.312 ng/ml. Sensitivity: 0.06 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human TRAF7 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

45594312

NP_115647.2

NM_032271.2

Q6Q0C0

74,609 Da

Tumor necrosis factor (TNF; see MIM 191160) receptor-associated factors, such as TRAF7, are signal transducers for members of the TNF receptor superfamily (see MIM 191190). TRAFs are composed of an N-terminal cysteine/histidine-rich region containing zinc RING and/or zinc finger motifs; a coiled-coil (leucine zipper) motif; and a homologous region that defines the TRAF family, the TRAF domain, which is involved in self-association and receptor binding.[supplied by OMIM, Apr 2004]

TRAF7: E3 ubiquitin ligase capable of auto-ubiquitination, following phosphorylation by MAP3K3. Potentiates MEKK3-mediated activation of the NF-kappa-B, JUN/AP1 and DDIT3 transcriptional regulators. Induces apoptosis when overexpressed. Belongs to the WD repeat TRAF7 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasmic membrane-bound vesicle; plasma membrane; nucleus; cytosol; ubiquitin ligase complex. Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity. Biological Process: positive regulation of protein sumoylation; regulation of protein localization; positive regulation of MAPKKK cascade; transcription, DNA-dependent; apoptosis; activation of MAPKKK activity; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter

48-Strip-Wells

275 USD

96-Strip-Wells

420