ELISA/assay

Human Tripartite motif-containing protein 3, TRIM3 ELISA Kit

MBS2602706

48-Strip-Wells

275 USD

ELISA Kit

Human Tripartite motif-containing protein 3, TRIM3 ELISA Kit

Tripartite motif-containing protein 3, TRIM3

tripartite motif-containing protein 3 isoform 1; Tripartite motif-containing protein 3; tripartite motif-containing protein 3; RING finger protein 97; ring finger protein 22; brain expressed ring finger; tripartite motif-containing 3; tripartite motif protein TRIM3; brain-expressed RING finger protein; tripartite motif containing 3; Brain-expressed RING finger protein; RING finger protein 22; RING finger protein 97

TRIM3

TRIM3; TRIM3; BERP; HAC1; RNF22; RNF97; BERP; RNF22; RNF97

TRIM3_HUMAN

Human

No cross-reaction with other factors.

Store all reagents at 2-8 degree C.

Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TRIM3 concentration. Assay Type: Sandwich. Detection Range: 10 ng/ml-0.156 ng/ml. Sensitivity: 0.05 ng/ml.

Intra-assay Precision: <= 8%. Inter-assay Precision: <= 12%

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human TRIM3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

351721563

NP_001234935.1

NM_001248006.1

O75382

80,830 Da

The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008]

TRIM3: Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Belongs to the TRIM/RBCC family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cytoskeletal; Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: cytoplasm; early endosome. Molecular Function: protein C-terminus binding; protein binding; zinc ion binding; ubiquitin-protein ligase activity. Biological Process: nervous system development; protein transport; protein ubiquitination

48-Strip-Wells

275 USD

96-Strip-Wells

420